Detection and typing of human papillomavirus in cutaneous warts of patients infected with human immunodeficiency virus type 1

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dc.contributor.authorPorro, A. M.
dc.contributor.authorAlchorne, MMA
dc.contributor.authorMota, G. R.
dc.contributor.authorMichalany, N.
dc.contributor.authorPignatari, ACC
dc.contributor.authorSouza, I. E.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:34:09Z
dc.date.available2016-01-24T12:34:09Z
dc.date.issued2003-12-01
dc.description.abstractBackground Cutaneous warts are caused by human papillomavirus (HPV). To date, more than 120 different types of HPV are known, of which 80 have been completely characterized. Prevalence studies on types of HPV present in cutaneous warts have been carried out in immunocompetent individuals and immunosuppressed organ allograft recipients, but not in human immunodeficiency virus (HIV)-positive patients.Objectives To determine the HPV types present in cutaneous warts of HIV-infected patients.Methods Twenty-five biopsies of cutaneous warts from HIV-infected patients and 14 samples from control non-HIV-infected patients were studied. HPV detection was performed by polymerase chain reaction using two sets of primers: MY09/MY11 and RK91. the type of HPV was determined by restriction fragment length polymorphism analysis and direct sequencing of the amplified products.Results HPV DNA was detected in 64% of cutaneous warts from HIV-infected patients and in 79% of samples from the control group. the HPV types identified in HIV-infected patients were: HPV 2 (38%), 57 (31%), 27 (12%), 6 (12%) and 7 (6%). HPV 2/27/57 predominated in both groups, being present in 81% of lesions from HIV-infected patients and 82% of samples from non-HIV-infected patients. HPV 6, a genital HPV type rarely found in cutaneous lesions, was detected in two warts from HIV-infected patients and in one lesion of the immunocompetent group. HPV 7, characteristically associated with butcher's warts, and recently detected in oral and perioral lesions of HIV-infected patients, was found for the first time in a non-facial lesion of an HIV-infected patient.Conclusions This is the first study evaluating the prevalence of HPV types in cutaneous warts of HIV-infected patients and immunocompetent individuals in Brazil.en
dc.description.affiliationUniversidade Federal de São Paulo, Sch Med, Infect Dis Unit, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Sch Med, Dept Dermatol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Sch Med, Dept Pathol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Sch Med, Infect Dis Unit, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Sch Med, Dept Dermatol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Sch Med, Dept Pathol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent1192-1199
dc.identifierhttp://dx.doi.org/10.1111/j.1365-2133.2003.05650.x
dc.identifier.citationBritish Journal of Dermatology. Oxford: Blackwell Publishing Ltd, v. 149, n. 6, p. 1192-1199, 2003.
dc.identifier.doi10.1111/j.1365-2133.2003.05650.x
dc.identifier.issn0007-0963
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/27509
dc.identifier.wosWOS:000187154400011
dc.language.isoeng
dc.publisherBlackwell Publishing Ltd
dc.relation.ispartofBritish Journal of Dermatology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectAIDSen
dc.subjectcutaneous wartsen
dc.subjecthuman immunodeficiency virusen
dc.subjecthuman papillomavirusen
dc.subjectpolymerase chain reactionen
dc.titleDetection and typing of human papillomavirus in cutaneous warts of patients infected with human immunodeficiency virus type 1en
dc.typeinfo:eu-repo/semantics/article
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