Endostatin neoadjuvant gene therapy extends survival in an orthotopic metastatic mouse model of renal cell carcinoma

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dc.contributor.authorBraga, Marina de Souza [UNIFESP]
dc.contributor.authorChaves, Karen Barbosa [UNIFESP]
dc.contributor.authorChammas, Roger [UNIFESP]
dc.contributor.authorSchor, Nestor [UNIFESP]
dc.contributor.authorBellini, Maria Helena [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionDept Biotechnol
dc.date.accessioned2016-01-24T14:27:19Z
dc.date.available2016-01-24T14:27:19Z
dc.date.issued2012-06-01
dc.description.abstractDespite recent advances in targeted therapy, renal cell carcinoma (RCC) remains one of the most lethal urologic malignancies. Approximately 30% of patients with localised RCC will develop metastases after curative surgery. Presurgical therapy has been explored for treatment of localised RCC. Endostatin (ES) is a fragment of collagen XVIII that possesses antiangiogenic activity. in this study, we examined the potential use of an antiangiogenic agent as a neoadjuvant therapy in an orthotopic metastatic mouse model of RCC. BALB/c mice bearing Renca cells were treated before nephrectomy with NIH/3T3-LendSN cells. At the end of the experiment, ES serum levels were measured. Primary and metastatic tumour area and microvascular area were determined. in the survival studies, mice were monitored daily until they died. ES serum levels in treated mice were higher in the control group (P < 0.05). the median primary tumour area and the mean microvascular area were significantly lower in the ES-treated group compared to control group (P < 0.05). the proliferation of Renca cells in the ES-treated group was significantly reduced compared with the control group (P < 0.01). ES therapy led to a significant reduction in the number of pulmonary metastatic nodules compared with the control group (P < 0.01). Kaplan-Meier survival curves showed that the probability of survival was significantly higher in mice receiving ES therapy (P = 0.0243, Log-Rank test). Our results indicated that neoadjuvant ES gene therapy has the potential to decrease tumour burden, extend survival, and may have clinical benefit in the management of RCC. (C) 2011 Elsevier Masson SAS. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Div Nephrol, Dept Med, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Radiol, São Paulo, Brazil
dc.description.affiliationDept Biotechnol, IPEN CNEN, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Nephrol, Dept Med, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Radiol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 2010/18969-0
dc.description.sponsorshipIDCAPES: 72-71859
dc.format.extent237-241
dc.identifierhttp://dx.doi.org/10.1016/j.biopha.2011.11.002
dc.identifier.citationBiomedicine & Pharmacotherapy. Paris: Elsevier France-editions Scientifiques Medicales Elsevier, v. 66, n. 4, p. 237-241, 2012.
dc.identifier.doi10.1016/j.biopha.2011.11.002
dc.identifier.issn0753-3322
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/34952
dc.identifier.wosWOS:000305811800001
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBiomedicine & Pharmacotherapy
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectEndostatinen
dc.subjectGene therapyen
dc.subjectNeoadjuvant therapyen
dc.subjectRetroviral vectoren
dc.titleEndostatin neoadjuvant gene therapy extends survival in an orthotopic metastatic mouse model of renal cell carcinomaen
dc.typeinfo:eu-repo/semantics/article
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