Assessment of the progressive nature of cell damage in the pilocarpine model of epilepsy

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dc.contributor.authorCovolan, Luciene [UNIFESP]
dc.contributor.authorMello, Luiz Eugenio Araujo de Moraes [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:36:19Z
dc.date.available2015-06-14T13:36:19Z
dc.date.issued2006-07-01
dc.description.abstractPilocarpine-induced (320 mg/kg, ip) status epilepticus (SE) in adult (2-3 months) male Wistar rats results in extensive neuronal damage in limbic structures. Here we investigated whether the induction of a second SE (N = 6) would generate damage and cell loss similar to that seen after a first SE (N = 9). Counts of silver-stained (indicative of cell damage) cells, using the Gallyas argyrophil III method, revealed a markedly lower neuronal injury in animals submitted to re-induction of SE compared to rats exposed to a single episode of pilocarpine-induced SE. This effect could be explained as follows: 1) the first SE removes the vulnerable cells, leaving behind resistant cells that are not affected by the second SE; 2) the first SE confers increased resistance to the remaining cells, analogous to the process of ischemic tolerance. Counting of Nissl-stained cells was performed to differentiate between these alternative mechanisms. Our data indicate that different neuronal populations react differently to SE induction. For some brain areas most, if not all, of the vulnerable cells are lost after an initial insult leaving only relatively resistant cells and little space for further damage or cell loss. For some other brain areas, in contrast, our data support the hypothesis that surviving cells might be modified by the initial insult which would confer a sort of excitotoxic tolerance. As a consequence of both mechanisms, subsequent insults after an initial insult result in very little damage regardless of their intensity.en
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Departamento de Fisiologia
dc.description.affiliationUnifespUNIFESP, Depto. de Fisiologia
dc.description.sourceSciELO
dc.format.extent915-924
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2006000700010
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 39, n. 7, p. 915-924, 2006.
dc.identifier.doi10.1590/S0100-879X2006000700010
dc.identifier.fileS0100-879X2006000700010.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2006000700010
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/3146
dc.identifier.wosWOS:000239624200010
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCell injuryen
dc.subjectStatus epilepticusen
dc.subjectEpilepsyen
dc.subjectPilocarpineen
dc.subjectSpontaneous seizuresen
dc.titleAssessment of the progressive nature of cell damage in the pilocarpine model of epilepsyen
dc.typeinfo:eu-repo/semantics/article
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