Analysis of HSPA8 and HSPA9 mRNA Expression and Promoter Methylation in the Brain and Blood of Alzheimer's Disease Patients

dc.contributor.authorSilva, Patricia Natalia [UNIFESP]
dc.contributor.authorFuruya, Tatiane Katsue [UNIFESP]
dc.contributor.authorBraga, Ianna Lacerda [UNIFESP]
dc.contributor.authorRasmussen, Lucas Trevizani
dc.contributor.authorLabio, Roger Willian
dc.contributor.authorBertolucci, Paulo Henrique [UNIFESP]
dc.contributor.authorChen, Elizabeth Suchi [UNIFESP]
dc.contributor.authorTurecki, Gustavo
dc.contributor.authorMechawar, Naguib
dc.contributor.authorPayao, Spencer Luiz [UNIFESP]
dc.contributor.authorMill, Jonathan
dc.contributor.authorSmith, Marilia Cardoso [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFac Med Marilia FAMEMA
dc.contributor.institutionMcGill Univ
dc.contributor.institutionKings Coll London
dc.date.accessioned2016-01-24T14:35:07Z
dc.date.available2016-01-24T14:35:07Z
dc.date.issued2014-01-01
dc.description.abstractAlzheimer's disease (AD) is the most common form of dementia in elderly. Chaperones may have a crucial role in AD due to their involvement in protein quality control, folding, and degradation. in this study, we investigated the mRNA and promoter DNA methylation levels of two chaperones, HSPA8 and HSPA9, in postmortem brain tissue (entorhinal and auditory cortices and hippocampus) from healthy elderly and AD subjects as well as in peripheral blood of healthy elderly and AD patients. mRNA quantification was performed by qRT-PCR and DNA methylation by mass spectrometry. in the peripheral blood, we did not observe a significant difference in HSPA8 and HSPA9 expression between elderly controls and AD. Asignificant downregulation of HSPA8 and HSPA9 was observed in AD across the three brain regions compared to the controls, suggesting their participation in AD pathogenesis. However, no important DNA methylation differences were observed, suggesting that other mechanism may be involved in controlling these genes expression.en
dc.description.affiliationUniversidade Federal de São Paulo UNIFESP, Disciplina Genet, Dept Morfol & Genet, São Paulo, Brazil
dc.description.affiliationDept Neurol & Neurocirurgia UNIFESP, Disciplina Neurol Clin, São Paulo, Brazil
dc.description.affiliationFac Med Marilia FAMEMA, Lab Genet, Hemoctr, Marilia, SP, Brazil
dc.description.affiliationMcGill Univ, Dept Psychiat, Douglas Hosp, Res Ctr, Montreal, PQ, Canada
dc.description.affiliationKings Coll London, Inst Psychiat, London, England
dc.description.affiliationUnifespUniversidade Federal de São Paulo UNIFESP, Disciplina Genet, Dept Morfol & Genet, São Paulo, Brazil
dc.description.affiliationUnifespDept Neurol & Neurocirurgia UNIFESP, Disciplina Neurol Clin, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent165-170
dc.identifierhttp://dx.doi.org/10.3233/JAD-130428
dc.identifier.citationJournal of Alzheimers Disease. Amsterdam: Ios Press, v. 38, n. 1, p. 165-170, 2014.
dc.identifier.doi10.3233/JAD-130428
dc.identifier.issn1387-2877
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/37299
dc.identifier.wosWOS:000326380300015
dc.language.isoeng
dc.publisherIos Press
dc.relation.ispartofJournal of Alzheimers Disease
dc.rightsAcesso restrito
dc.rights.licensehttp://www.iospress.nl/service/authors/author-copyright-agreement/
dc.subjectepigeneticsen
dc.subjectgene expressionen
dc.subjectHSPA8en
dc.subjectHSPA9en
dc.subjectmRNAen
dc.titleAnalysis of HSPA8 and HSPA9 mRNA Expression and Promoter Methylation in the Brain and Blood of Alzheimer's Disease Patientsen
dc.typeArtigo
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