A transcript finishing initiative for closing gaps in the human transcriptome

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dc.contributor.authorSogayar, Mari Cleide
dc.contributor.authorCamargo, Anamaria Aranha
dc.contributor.authorBettoni, Fabiana
dc.contributor.authorCarraro, Dirce Maria
dc.contributor.authorPires, Lilian C.
dc.contributor.authorParmigiani, Raphael B.
dc.contributor.authorFerreira, Elisa N.
dc.contributor.authorMoreira, Eloisa de Sá
dc.contributor.authorLatorre, Maria do Rosário Dias de Oliveira
dc.contributor.authorSimpson, Andrew John George
dc.contributor.authorCruz, Luciana Oliveira
dc.contributor.authorDegaki, Theri Leica
dc.contributor.authorFesta, Fernanda
dc.contributor.authorMassirer, Katlin Brauer
dc.contributor.authorCamargo Filho, Fernando
dc.contributor.authorCamargo, Luiz Paulo
dc.contributor.authorCunha, Marco A. V.
dc.contributor.authorSouza, Sandro José de
dc.contributor.authorFaria Junior, Milton
dc.contributor.authorGiuliatti, Silvana
dc.contributor.authorKopp, Leonardo
dc.contributor.authorOliveira, Paulo Sergio Lopes de
dc.contributor.authorPaiva, Paulo Bandiera [UNIFESP]
dc.contributor.authorPereira, Anderson Alvarenga
dc.contributor.authorPinheiro, Daniel G.
dc.contributor.authorPuga, Renato David
dc.contributor.authorSouza, Jorge Estefano Santana de
dc.contributor.authorAlbuquerque, Dulcineia M.
dc.contributor.authorAndrade, Luiz Eduardo Coelho [UNIFESP]
dc.contributor.authorBaia, Gilson Soares
dc.contributor.authorBriones, Marcelo Ribeiro da Silva [UNIFESP]
dc.contributor.authorCavaleiro-Luna, Ana M. S.
dc.contributor.authorCerutti, Janete Maria [UNIFESP]
dc.contributor.authorCosta, Fernando Ferreira
dc.contributor.authorCostanzi-Strauss, Eugenia [UNIFESP]
dc.contributor.authorEspreafico, Enilza M.
dc.contributor.authorFerrasi, Adriana C.
dc.contributor.authorFerro, Emer Suavinho
dc.contributor.authorFortes, Maria A. H. Z.
dc.contributor.authorFurchi, Joelma R. F.
dc.contributor.authorGianella-Neto, Daniel
dc.contributor.authorGoldman, Gustavo Henrique
dc.contributor.authorGoldman, Maria Helena S.
dc.contributor.authorGruber, Arthur
dc.contributor.authorGuimaraes, Gustavo Stuani
dc.contributor.authorHackel, Christine
dc.contributor.authorHenrique-Silva, Flavio
dc.contributor.authorKimura, Edna Teruko
dc.contributor.authorLeoni, Suzana G.
dc.contributor.authorMacedo, Claudia
dc.contributor.authorMalnic, Bettina
dc.contributor.authorManzini, Carina V.
dc.contributor.authorMarie, Suely Kazue Nagahashi
dc.contributor.authorMartinez-Rossi, Nilce M.
dc.contributor.authorMenossi, Marcelo
dc.contributor.authorMiracca, Elisabete C.
dc.contributor.authorNagai, Maria Aparecida
dc.contributor.authorNobrega, Francisco G.
dc.contributor.authorNobrega, Marina Pasetto
dc.contributor.authorOba-Shinjo, Sueli Mieko
dc.contributor.authorOliveira, Márika K.
dc.contributor.authorOrabona, Guilherme M.
dc.contributor.authorOtsuka, Audrey Yumi
dc.contributor.authorPaco-Larson, Maria L.
dc.contributor.authorPaixao, Beatriz M. C.
dc.contributor.authorPandolfi, José Rodrigo C.
dc.contributor.authorPardini, Maria Inês de Moura Campos
dc.contributor.authorPassos-Bueno, Maria Rita
dc.contributor.authorPassos, Geraldo Aleixo [UNIFESP]
dc.contributor.authorPesquero, João Bosco [UNIFESP]
dc.contributor.authorPessoa, Juliana Gilbert [UNIFESP]
dc.contributor.authorRahal, Paula
dc.contributor.authorRainho, Claudia Aparecida
dc.contributor.authorReis, Caroline P.
dc.contributor.authorRicca, Tatiana Iervolino [UNIFESP]
dc.contributor.authorRodriguez, Vanderlei
dc.contributor.authorRogatto, Silvia Regina
dc.contributor.authorRomano, Camila M.
dc.contributor.authorRomeiro, Janaina G.
dc.contributor.authorRossi, Antonio
dc.contributor.authorSá, Renata G.
dc.contributor.authorSales, Magaly M.
dc.contributor.authorSant'Anna, Simone C.
dc.contributor.authorSantarosa, Patricia L.
dc.contributor.authorSegato, Fernando
dc.contributor.authorSilva Junior, Wilson. Araujo
dc.contributor.authorSilva, Ismael Dale Cotrim Guerreiro da [UNIFESP]
dc.contributor.authorSilva, Neusa Pereira da [UNIFESP]
dc.contributor.authorSoares-Costa, Andrea
dc.contributor.authorSonati, Maria de Fátima
dc.contributor.authorStrauss, Bryan Eric
dc.contributor.authorTajara, Eloiza Helena
dc.contributor.authorValentini, Sandro Roberto
dc.contributor.authorVillanova, Fabiula Elizabeth [UNIFESP]
dc.contributor.authorWard, Laura Sterian
dc.contributor.authorZanette, Dalila L.
dc.contributor.authorLudwig-FAPESP Transcript Finishing In
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionLudwig Inst Canc Res
dc.contributor.institutionUniv Ribeirao Preto
dc.contributor.institutionInst Ludwig
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniv Vale Paraiba
dc.contributor.institutionSão Paulo State Univ
dc.contributor.institutionUniv Estadual Paulista
dc.date.accessioned2016-01-24T12:37:13Z
dc.date.available2016-01-24T12:37:13Z
dc.date.issued2004-07-01
dc.description.abstractWe report the results of a transcript finishing initiative, undertaken for the purpose of identifying and characterizing novel human transcripts, in which RT-PCR was used to bridge gaps between paired EST Clusters, mapped against the genomic sequence. Each pair of EST Clusters selected for experimental validation was designated a transcript finishing unit (TFU). A total of 489 TFUs were selected for validation, and an overall efficiency of 43.1% was achieved. We generated a total of 59,975 bp of transcribed sequences organized into 432 exons, contributing to the definition of the structure of 211 human transcripts. the structure of several transcripts reported here was confirmed during the course of this project, through the generation of their corresponding full-length cDNA sequences. Nevertheless, for 21% of the validated TFUs, a full-length cDNA sequence is not yet available in public databases, and the structure of 69.2% of these TFUs was not correctly predicted by computer programs. the TF strategy provides a significant contribution to the definition of the complete catalog of human genes and transcripts, because it appears to be particularly useful for identification of low abundance transcripts expressed in a restricted Set of tissues as well as for the delineation of gene boundaries and alternatively spliced isoforms.en
dc.description.affiliationUniv São Paulo, Inst Quim, BR-05513970 São Paulo, Brazil
dc.description.affiliationLudwig Inst Canc Res, BR-01509010 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Epidemiol, Sch Publ Hlth, BR-01246904 São Paulo, SP, Brazil
dc.description.affiliationUniv Ribeirao Preto, Dept Quim Informat Bioinformat, BR-14096380 Ribeirao Preto, Brazil
dc.description.affiliationUniv São Paulo, Fac Med Ribeirao Preto, Centro Terapia Celular, Hemocentro, BR-14051140 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv São Paulo, Fac Med Ribeirao Preto, Dept Clin Med, BR-14051140 Ribeirao Preto, SP, Brazil
dc.description.affiliationInst Ludwig, Lab Biol Computacional, BR-01509010 São Paulo, SP, Brazil
dc.description.affiliationUniv São Paulo, Lab Genet Cardiol Mol, Inst Coracao, BR-05403000 São Paulo, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Bioinform Lab, Hlth Informatics Dept, BR-04039032 São Paulo, Brazil
dc.description.affiliationUniv Estad Campinas, Dept Clin Med, Fac Ciencias Med, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Rheumatol Div, BR-04113001 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Histol Embriol, Inst Ciencias Biomed, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Immunol Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Lab Cellular Mol Endocrinol, Sch Med, BR-01246903 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Med, Disciplina Endocrinol, Lab Endocrinol Mol, BR-04039002 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Ciencias Biomed, Lab Transferencia Genica, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med Ribeirao Preto, Dept Biol Celular Mol Bioagentes Patogenicas, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Lab Biol Mol, Hemocentro, Fac Med, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUniv Fed Sao Carlos, Dept Genet Evol, BR-13565905 Sao Carlos, SP, Brazil
dc.description.affiliationUniv São Paulo, Fac Ciencias Farmaceuticas Ribeirao Preto, BR-14040903 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv São Paulo, Fac Filosofia Ciencias Letras Ribeirao Preto, Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Patol, Fac Med Vet Zootecnia, BR-05508000 São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Dept Genet Med, Fac Ciencias Med, BR-13081970 Campinas, SP, Brazil
dc.description.affiliationUniv São Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14040900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Bioquim, Inst Quim, BR-05599970 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Neurol, Fac Med, BR-01246903 São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Lab Genoma Funcional, Centro Biol Mol Engenharia Genet, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Dept Genet Evol, Inst Biol, Campinas, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Radiol, Disciplina Oncol, Fac Med, BR-01246903 São Paulo, Brazil
dc.description.affiliationUniv Vale Paraiba, Inst Pesquisa Desenvolvimento, BR-12244000 Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUniv São Paulo, Inst Biociencias, Centro Estudios Genoma Humano, Dept Biol, BR-055508900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Lab Mol Gynecol, Dept Gynecol, BR-04039001 São Paulo, Brazil
dc.description.affiliationSão Paulo State Univ, Sch Pharmacol, Dept Biol Sci, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUniv São Paulo, Discipl Genet, Fac Odontol, BR-14040900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biofisica, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Biol, Inst Biociencias Letras Ciencias Exatas, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Genet, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Bioquim Imunol, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Lab Genet Mol Cancer, Dept Clin Med, Fac Ciencias Med, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Dept Patol Clin, Fac Ciencias Med, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv São Paulo, Setor Vetores Virals, Lab Cardiol Mol, Inst Coracao, BR-15403000 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Bioinform Lab, Hlth Informatics Dept, BR-04039032 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Rheumatol Div, BR-04113001 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Immunol Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Med, Disciplina Endocrinol, Lab Endocrinol Mol, BR-04039002 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Lab Mol Gynecol, Dept Gynecol, BR-04039001 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biofisica, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent1413-1423
dc.identifierhttps://dx.doi.org/10.1101/gr.2111304
dc.identifier.citationGenome Research. Cold Spring Harbor: Cold Spring Harbor Lab Press, Publications Dept, v. 14, n. 7, p. 1413-1423, 2004.
dc.identifier.doi10.1101/gr.2111304
dc.identifier.fileWOS000222434200023.pdf
dc.identifier.issn1088-9051
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/27803
dc.identifier.wosWOS:000222434200023
dc.language.isoeng
dc.publisherCold Spring Harbor Lab Press, Publications Dept
dc.relation.ispartofGenome Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleA transcript finishing initiative for closing gaps in the human transcriptomeen
dc.typeinfo:eu-repo/semantics/article
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