Cardioprotective mechanism of S-nitroso-N-acetylcysteine via S-nitrosated betadrenoceptor-2 in the LDLr-/- mice

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dc.contributor.authorWanschel, Amarylis Claudine Bonito Azeredo
dc.contributor.authorCaceres, Viviane de Menezes [UNIFESP]
dc.contributor.authorMoretti, Ana Iochabel Soares
dc.contributor.authorBruni-Cardoso, Alexandre
dc.contributor.authorCarvalho, Hernandes Faustino de
dc.contributor.authorSouza, Heraldo Possolo de
dc.contributor.authorLaurindo, Francisco Rafael Martins
dc.contributor.authorSpadari, Regina Celia [UNIFESP]
dc.contributor.authorKrieger, Marta Helena
dc.contributor.institutionNYU
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T14:35:10Z
dc.date.available2016-01-24T14:35:10Z
dc.date.issued2014-01-30
dc.description.abstractPrevious studies from our group have demonstrated the protective effect of S-nitroso-N-acetylcysteine (SNAC) on the cardiovascular system in dyslipidemic LDLr-/- mice that develop atheroma and left ventricular hypertrophy after 15 days on a high fat diet. We have shown that SNAC treatment attenuates plaque development via the suppression of vascular oxidative stress and protects the heart from structural and functional myocardial alterations, such as heart arrhythmia, by reducing cardiomyocyte sensitivity to catecholamines. Here we investigate the ability of SNAC to modulate oxidative stress and cell survival in cardiomyocytes during remodeling and correlation with beta(2)-AR signaling in mediating this protection. Ventricular superoxide (O-2(-)) and hydrogen peroxide (H2O2) generation was measured by HPLC methods to allow quantification of dihydroethidium (DHE) products. Ventricular histological sections were stained using terminal dUTP nick-end labeling (TUNEL) to identify nuclei with DNA degradation (apoptosis) and this was confirmed by Western blot for cleaved caspase-3 and caspase-7 protein expression. the findings show that O-2(-) and H2O2 production and also cell apoptosis were increased during left ventricular hypertrophy (LVH). SNAC treatment reduced oxidative stress during on cardiac remodeling, measured by decreased H2O2 and O-2(-) production (65% and 52%, respectively), and a decrease in the ratio of p-Ser1177 eNOS/total eNOS. Left ventricle (LV) from SNAC-treated mice revealed a 4-fold increase in beta(2)-AR expression associated with coupling change to Gi; beta(2)-ARs-S-nitrosation (beta(2)-AR-SNO) increased 61%, while apoptosis decreased by 70%. These results suggest that the cardio-protective effect of SNAC treatment is primarily through its anti-oxidant role and is associated with beta(2)-ARs overexpression and beta(2)-AR-SNO via an anti-apoptotic pathway. (C) 2013 Elsevier Inc. All rights reserved.en
dc.description.affiliationNYU, Sch Med, Marc & Ruti Bell Vasc Biol & Dis Program, Leon H Charney Div Cardiol,Dept Med, New York, NY 10016 USA
dc.description.affiliationState Univ Campinas UNICAMP, Inst Biol, Dept Anat Cellular Biol & Physiol, São Paulo, Brazil
dc.description.affiliationFed Univ São Paulo UNIFESP, Dept Biosci, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Emergency Med, BR-09500900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Sch Med, Heart Inst InCor, BR-09500900 São Paulo, Brazil
dc.description.affiliationUnifespFed Univ São Paulo UNIFESP, Dept Biosci, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent58-66
dc.identifierhttp://dx.doi.org/10.1016/j.niox.2013.12.003
dc.identifier.citationNitric Oxide-biology and Chemistry. San Diego: Academic Press Inc Elsevier Science, v. 36, p. 58-66, 2014.
dc.identifier.doi10.1016/j.niox.2013.12.003
dc.identifier.fileWOS000330605700008.pdf
dc.identifier.issn1089-8603
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/37333
dc.identifier.wosWOS:000330605700008
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofNitric Oxide-biology and Chemistry
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectHydrogen peroxideen
dc.subjectSuperoxideen
dc.subjectS-nitroso-N-acetylscysteineen
dc.subjectVentricular hypertrophyen
dc.subjectBetadrenoceptor-2en
dc.subjectS-nitrosateden
dc.titleCardioprotective mechanism of S-nitroso-N-acetylcysteine via S-nitrosated betadrenoceptor-2 in the LDLr-/- miceen
dc.typeinfo:eu-repo/semantics/article
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