Consequences of pilocarpine-induced status epilepticus in immunodeficient mice

Página do item simplificado
dc.contributor.authorVignoli, Thiago [UNIFESP]
dc.contributor.authorNehlig, Astrid
dc.contributor.authorMassironi, Silvia Gomes
dc.contributor.authorSinigaglia Coimbra, Rita de Cassia [UNIFESP]
dc.contributor.authorNaffah Mazzacoratti, Maria da Graca [UNIFESP]
dc.contributor.authorSilva, Iara Ribeiro [UNIFESP]
dc.contributor.authorCastro Neto, Eduardo Ferreira de [UNIFESP]
dc.contributor.authorPersike, Daniele Suzete [UNIFESP]
dc.contributor.authorSilva Fernandes, Maria Jose da [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Strasbourg
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T14:27:07Z
dc.date.available2016-01-24T14:27:07Z
dc.date.issued2012-04-23
dc.description.abstractSystemic injection of pilocarpine in rodents induces status epilepticus (SE) and reproduces the main characteristics of temporal lobe epilepsy (TLE). Different mechanisms are activated by SE contributing to cell death and immune system activation. We used BALB/c nude mice, a mutant that is severely immunocompromised, to characterize seizure pattern, neurochemical changes, cell death and c-Fos activation secondarily to pilocarpine-induced SE. the behavioral seizures were less severe in BALB/c nude than in BALB/c wild type mice. However, nude mice presented more tonic clonic episodes and higher mortality rate during SE. the c-Fos expression was most prominent in the caudate-putamen, CA3 (p < 0.05), dentate gyrus, entorhinal cortex (p < 0.001), basolateral nucleus of amygdala (p < 0.01) and piriform cortex (p < 0.05) of BALB/c nude mice than of BALB/c. Besides, nude mice subjected to SE presented high number of Fluorojade-B (FJB) stained cells in the piriform cortex, amygdala (p < 0.05) and hilus (p < 0.05) in comparison with BALB/c mice. A significant increase in the level of glutamate and GABA was found in the hippocampus and cortex of BALB/c mice presenting SE in comparison to controls. However, the level of glutamate was higher in the brains of BALB nude mice than in the brains of BALB/c wild type mice, while the levels of GABA were unchanged. These results indicate that the brains of immunodeficient nude mice are more vulnerable to the deleterious effects of pilocarpine-induced SE as they present intense activation, increased glutamate levels and more cell death. Published by Elsevier B.V.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Neurol & Nurocirurgia, BR-04039032 São Paulo, SP, Brazil
dc.description.affiliationUniv Strasbourg, INSERM, Unite 666, Strasbourg, France
dc.description.affiliationUniv São Paulo, Dept Imunol, BR-05508 São Paulo, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Ctr Microscopia Elet, São Paulo, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Bioquim, BR-04039032 São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Neurol & Nurocirurgia, BR-04039032 São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Ctr Microscopia Elet, São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Bioquim, BR-04039032 São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipINNT/MCT
dc.format.extent125-137
dc.identifierhttp://dx.doi.org/10.1016/j.brainres.2012.02.028
dc.identifier.citationBrain Research. Amsterdam: Elsevier B.V., v. 1450, p. 125-137, 2012.
dc.identifier.doi10.1016/j.brainres.2012.02.028
dc.identifier.issn0006-8993
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/34803
dc.identifier.wosWOS:000303224600013
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBrain Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectBALB/c nude miceen
dc.subjectStatus epilepticusen
dc.subjectPilocarpineen
dc.subjectc-Fos, amino aciden
dc.subjectCell deathen
dc.titleConsequences of pilocarpine-induced status epilepticus in immunodeficient miceen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
Em verificação - Geral