Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejection

dc.contributor.authorShulzhenko, Natalia [UNIFESP]
dc.contributor.authorMorgun, Andrey [UNIFESP]
dc.contributor.authorFranco, Marcello Fabiano de [UNIFESP]
dc.contributor.authorSouza, Marcia Marcelino de [UNIFESP]
dc.contributor.authorAlmeida, Dirceu Rodrigues de [UNIFESP]
dc.contributor.authorDiniz, Rosiane Viana Zuza [UNIFESP]
dc.contributor.authorCarvalho, Antonio Carlos [UNIFESP]
dc.contributor.authorPacheco-Silva, Alvaro [UNIFESP]
dc.contributor.authorGerbase-Lima, Maria [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:29:26Z
dc.date.available2015-06-14T13:29:26Z
dc.date.issued2001-06-01
dc.description.abstractThe purpose of the present study was to investigate the expression (mRNA) of CD40 ligand (CD40L), interferon-gamma (IFN-gamma) and Fas ligand (FasL) genes in human cardiac allografts in relation to the occurrence of acute cardiac allograft rejection as well as its possible value in predicting acute rejection. The mRNA levels were determined by a semiquantitative reverse transcriptase-polymerase chain reaction method in 39 samples of endomyocardial biopsies obtained from 10 adult cardiac transplant recipients within the first six months after transplantation. Biopsies with ongoing acute rejection showed significantly higher CD40L, IFN-gamma and FasL mRNA expression than biopsies without rejection. The median values of mRNA expression in biopsies with and without rejection were 0.116 and zero for CD40L (P<0.003), 0.080 and zero for IFN-gamma (P<0.0009), and 0.156 and zero for FasL (P<0.002), respectively. In addition, the levels of IFN-gamma mRNA were significantly increased 7 to 15 days before the appearance of histological evidence of rejection (median of 0.086 in pre-rejection biopsies), i.e., they presented a predictive value. This study provides further evidence of heightened expression of immune activation genes during rejection and shows that some of these markers may present predictive value for the occurrence of acute rejection.en
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Departamento de Pediatria
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Departamento de Patologia
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Medicina
dc.description.affiliationUnifespUNIFESP, Depto. de Pediatria
dc.description.affiliationUnifespUNIFESP, Depto. de Patologia
dc.description.affiliationUnifespUNIFESP, EPM, Depto. de Medicina
dc.description.sourceSciELO
dc.format.extent779-784
dc.identifierhttps://dx.doi.org/10.1590/S0100-879X2001000600013
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 6, p. 779-784, 2001.
dc.identifier.doi10.1590/S0100-879X2001000600013
dc.identifier.fileS0100-879X2001000600013.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2001000600013
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/1190
dc.identifier.wosWOS:000169443400013
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsAcesso aberto
dc.subjectRejectionen
dc.subjectCardiac allograften
dc.subjectImmunologic monitoringen
dc.subjectmRNAen
dc.subjectGene activationen
dc.subjectInterferon-gammaen
dc.titleExpression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejectionen
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