Effectiveness, against tuberculosis, of pseudo-ternary complexes: Peptide-DNA-cationic liposome

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dc.contributor.authorRosada, Rogério Silva
dc.contributor.authorSilva, Cello Lopes
dc.contributor.authorSantana, Maria Helena Andrade
dc.contributor.authorNakaie, Clovis Ryuichi [UNIFESP]
dc.contributor.authorDe la Torre, Lucimara Gaziola
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:27:08Z
dc.date.available2016-01-24T14:27:08Z
dc.date.issued2012-05-01
dc.description.abstractWe report the effects of a synthetic peptide designed to act as a nuclear localization signal on the treatment of tuberculosis. the peptide contains 21 amino acid residues with the following specific domains: nuclear localization signal from SV 40T, cationic shuttle sequence, and cysteamide group at the C-terminus. the peptide was complexed with the plasmid DNAhsp65 and incorporated into cationic liposomes, forming a pseudo-ternary complex. the same cationic liposomes, composed of egg chicken L-alpha-phosphatidylcholine, 1,2-dioleoyl-3-trimethylammonium-propane, and 1,2-dioleoyl-3-trimethylammonium-propane (2:1:1 M), were previously evaluated as a gene carrier for tuberculosis immunization protocols with DNAhsp65. the pseudo-ternary complex presented a controlled size (250 nm), spherical-like shape, and various lamellae in liposomes as evaluated by transmission electron microscopy. An assay of fluorescence probe accessibility confirmed insertion of the peptide/DNA into the liposome structure. Peptide addition conferred no cytotoxicity in vitro, and similar therapeutic effects against tuberculosis were seen with four times less DNA compared with naked DNA treatment. Taken together, the results indicate that the pseudo-ternary complex is a promising gene vaccine for tuberculosis treatment. This work contributes to the development of multifunctional nanostructures in the search for strategies for in vivo DNA delivery. (C) 2011 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniv Estadual Campinas, Dept Proc Biotecnol, Fac Engn Quim, UNICAMP, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv São Paulo, Fac Med Ribeirao Preto, Nucleo Pesquisas TB, BR-14049900 São Paulo, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biofis, Escola Paulista Med, BR-04044020 São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biofis, Escola Paulista Med, BR-04044020 São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent102-109
dc.identifierhttp://dx.doi.org/10.1016/j.jcis.2011.09.040
dc.identifier.citationJournal of Colloid and Interface Science. San Diego: Academic Press Inc Elsevier Science, v. 373, p. 102-109, 2012.
dc.identifier.doi10.1016/j.jcis.2011.09.040
dc.identifier.fileWOS000302208300012.pdf
dc.identifier.issn0021-9797
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/34813
dc.identifier.wosWOS:000302208300012
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Colloid and Interface Science
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectCationic liposomeen
dc.subjectDNA deliveryen
dc.subjectNuclear localization signalen
dc.subjectTuberculosisen
dc.subjectGene vaccineen
dc.subjectInfectious diseaseen
dc.titleEffectiveness, against tuberculosis, of pseudo-ternary complexes: Peptide-DNA-cationic liposomeen
dc.typeinfo:eu-repo/semantics/article
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