Sensitivity of Salmonella YG5161 for Detecting PAH-Associated Mutagenicity in Air Particulate Matter

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dc.contributor.authorUmbuzeiro, Gisela A.
dc.contributor.authorKummrow, Fabio [UNIFESP]
dc.contributor.authorMorales, Daniel Alexandre
dc.contributor.authorAlves, Debora Kristina M.
dc.contributor.authorLim, Hwanmi
dc.contributor.authorJarvis, Ian W. H.
dc.contributor.authorBergvall, Christoffer
dc.contributor.authorWesterholm, Roger
dc.contributor.authorStenius, Ulla
dc.contributor.authorDreij, Kristian
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionStockholm Univ
dc.contributor.institutionKarolinska Inst
dc.date.accessioned2016-01-24T14:37:30Z
dc.date.available2016-01-24T14:37:30Z
dc.date.issued2014-07-01
dc.description.abstractThe Salmonella/microsome assay is the most used assay for the evaluation of air particulate matter (PM) mutagenicity and a positive correlation between strain TA98 responses and benzo[a]pyrene (B[a]P) levels in PM has been found. However, it seems that the major causes of PM mutagenicity in this assay are the nitro and oxy-PAHs. Salmonella YG5161, a 30-times more responsive strain to B[a]P has been developed. To verify if YG5161 strain was sufficiently sensitive to detect mutagenicity associated with B[a]P mutagenicity, PM samples were collected in Brazil and Sweden, extracted with toluene and tested in the Salmonella/microsome microsuspension assay. PAHs and B[a]P were determined and the extracts were tested with YG5161 and its parental strain TA1538. the extracts were also tested with YG1041 and its parental strain TA98. for sensitivity comparisons, we tested B[a]P and 1-nitropyrene (1-NP) using the same conditions. the minimal effective dose of B[a]P was 155 ng/plate for TA1538 and 7 ng/plate for YG5161. Although the maximum tested dose, 10 m(3)/plate containing 9 ng of B[a]P in the case of Brazilian sample, was sufficient to elicit a response in YG5161, mutagenicity was detected at a dose as low as 1 m(3)/plate (0.9 ng). This is probably caused by nitro-compounds that have been shown to be even more potent than B[a]P for YG5161. It seems that the mutagenicity of B[a]P present in PM is not detectable even with the use of YG5161 unless more efficient separation to remove the nitro-compounds from the PAH extract is performed. (C) 2014 Wiley Periodicals, Inc.en
dc.description.affiliationState Univ Campinas Unicamp, Fac Technol, Limeira, SP, Brazil
dc.description.affiliationFed Univ São Paulo Unifesp, Inst Environm Chem & Pharmaceut Sci, Diadema, SP, Brazil
dc.description.affiliationUniv São Paulo, Fac Pharmaceut Sci, São Paulo, Brazil
dc.description.affiliationStockholm Univ, Dept Analyt Chem, S-10691 Stockholm, Sweden
dc.description.affiliationKarolinska Inst, Inst Environm Med, S-10401 Stockholm, Sweden
dc.description.affiliationUnifespFed Univ São Paulo Unifesp, Inst Environm Chem & Pharmaceut Sci, Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipSwedish Research Council Formas
dc.description.sponsorshipCancer-och Allergi-fonden
dc.description.sponsorshipStockholm University
dc.format.extent510-517
dc.identifierhttp://dx.doi.org/10.1002/em.21861
dc.identifier.citationEnvironmental and Molecular Mutagenesis. Hoboken: Wiley-Blackwell, v. 55, n. 6, p. 510-517, 2014.
dc.identifier.doi10.1002/em.21861
dc.identifier.issn0893-6692
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/37917
dc.identifier.wosWOS:000339108600007
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofEnvironmental and Molecular Mutagenesis
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectPAHsen
dc.subjectsalmonella/microsomeen
dc.subjectnitro-PAHsen
dc.subjectmutagenicityen
dc.subjectair pollutionen
dc.titleSensitivity of Salmonella YG5161 for Detecting PAH-Associated Mutagenicity in Air Particulate Matteren
dc.typeinfo:eu-repo/semantics/article
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