Involvement of the major glycoprotein (gp43) of Paracoccidioides brasiliensis in attachment to macrophages

dc.contributor.authorAlmeida, SR
dc.contributor.authorUnterkircher, C. S.
dc.contributor.authorCamargo, Z. P.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUNESP
dc.date.accessioned2016-01-24T12:30:43Z
dc.date.available2016-01-24T12:30:43Z
dc.date.issued1998-12-01
dc.description.abstractThe yeast form of Paracoccidioides brasiliensis, the causative agent of a deep mycosis in humans, is known to be phagocytized by, and to multiply inside, macrophages. in this work we describe the involvement of gp43, a major antigenic protein of P. brasiliensis, in the initial steps of attachment of the fungus to macrophages. Anti-gp43 F(ab) polyclonal fragments were capable of inhibiting phagocytosis in a concentration-dependent manner. Sheep red blood cells sensitized with purified gp43 were more endocytized than SF:BC alone, and this process was also inhibited by anti-gp43 F(ab) fragments. Inhibition tests indicated the involvement of fucose and mannose residues in the phagocytosis of the fungus and of SRBC-gp43 by macrophages. Taken together, these results suggest that gp43 may be involved in the adherence and uptake of the fungus by murine peritoneal macrophages, and that this binding may be dependent on monosaccharide residues that are part of the gp43 glycoprotein.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUNESP, Sch Dent, Dept Pathol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent405-411
dc.identifierhttp://dx.doi.org/10.1080/02681219880000641
dc.identifier.citationMedical Mycology. Oxford: Blackwell Science Ltd, v. 36, n. 6, p. 405-411, 1998.
dc.identifier.doi10.1080/02681219880000641
dc.identifier.issn1369-3786
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/25993
dc.identifier.wosWOS:000077858400008
dc.language.isoeng
dc.publisherBlackwell Science Ltd
dc.relation.ispartofMedical Mycology
dc.rightsAcesso restrito
dc.subjectmacrophagesen
dc.subjectParacoccidioides brasiliensisen
dc.subjectparacoccidioidomycosisen
dc.subjectphagocytosisen
dc.titleInvolvement of the major glycoprotein (gp43) of Paracoccidioides brasiliensis in attachment to macrophagesen
dc.typeArtigo
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