Lipophosphoglycans from Leishmania amazonensis Strains Display Immunomodulatory Properties via TLR4 and Do Not Affect Sand Fly Infection

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dc.citation.issue8
dc.citation.volume10
dc.contributor.authorNogueira, Paula M.
dc.contributor.authorAssis, Rafael R.
dc.contributor.authorTorrecilhas, Ana C. [UNIFESP]
dc.contributor.authorSaraiva, Elvira M.
dc.contributor.authorPessoa, Natlia L.
dc.contributor.authorCampos, Marco A.
dc.contributor.authorMarialva, Eric F.
dc.contributor.authorRios-Velasquez, Claudia M.
dc.contributor.authorPessoa, Felipe A.
dc.contributor.authorSecundino, Nagila F.
dc.contributor.authorRugani, Jeronimo N.
dc.contributor.authorNieves, Elsa
dc.contributor.authorTurco, Salvatore J.
dc.contributor.authorMelo, Maria N.
dc.contributor.authorSoares, Rodrigo P.
dc.coverageSan Francisco
dc.date.accessioned2020-08-14T13:43:58Z
dc.date.available2020-08-14T13:43:58Z
dc.date.issued2016
dc.description.abstractThe immunomodulatory properties of lipophosphoglycans (LPG) from New World species of Leishmania have been assessed in Leishmania infantum and Leishmania braziliensis, the causative agents of visceral and cutaneous leishmaniasis, respectively. This glycoconjugate is highly polymorphic among species with variation in sugars that branch off the conserved Gal(beta 1,4) Man(alpha 1)-PO4 backbone of repeat units. Here, the immunomodulatory activity of LPGs from Leishmania amazonensis, the causative agent of diffuse cutaneous leishmaniasis, was evaluated in two strains from Brazil. One strain (PH8) was originally isolated from the sand fly and the other (Josefa) was isolated from a human case. The ability of purified LPGs from both strains was investigated during in vitro interaction with peritoneal murine macrophages and CHO cells and in vivo infection with Lutzomyia migonei. In peritoneal murine macrophages, the LPGs from both strains activated TLR4. Both LPGs equally activate MAPKs and the NF-kappa B inhibitor p-I.Ba, but were not able to translocate NF-kappa B. In vivo experiments with sand flies showed that both stains were able to sustain infection in L. migonei. A preliminary biochemical analysis indicates intraspecies variation in the LPG sugar moieties. However, they did not result in different activation profiles of the innate immune system. Also those polymorphisms did not affect infectivity to the sand fly.en
dc.description.affiliationFiocruz MS, Ctr Pesquisas Rene Rachou, Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Fed Minas Gerais, Dept Parasitol, Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Lab Imunol Celular & Bioquim Fungos & Protozoanos, Dept Ciencias Biol, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Rio de Janeiro, Lab Imunobiol Leishmanioses, Dept Imunol, Rio de Janeiro, Brazil
dc.description.affiliationFiocruz MS, Ctr Pesquisas Leonidas & Maria Deane, Manaus, Amazonas, Brazil
dc.description.affiliationUniv Los Andes, Dept Biol, Lab Parasitol Expt, Merida, Venezuela
dc.description.affiliationUniv Kentucky, Med Ctr, Dept Biochem, Lexington, KY 40536 USA
dc.description.affiliationUnifespUniv Fed Sao Paulo, Lab Imunol Celular & Bioquim Fungos & Protozoanos, Dept Ciencias Biol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Pesquisa e Desenvolvimento of Brazil
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipIDCNPq PAPES VI: 407438/2012-2
dc.description.sponsorshipIDCNPq PAPES VI: 407687/2012-2
dc.description.sponsorshipIDCNPq PAPES VI: 407743/2012
dc.description.sponsorshipIDCNPq PAPES VI: 168260/2014-0
dc.description.sponsorshipIDCNPq PROEP: 401975/2012
dc.description.sponsorshipIDFAPEMIG: PPM-00163-14
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1371/journal.pntd.0004848
dc.identifier.citationPlos Neglected Tropical Diseases. San Francisco, v. 10, n. 8, p. -, 2016.
dc.identifier.doi10.1371/journal.pntd.0004848
dc.identifier.fileWOS000382390800010.pdf
dc.identifier.issn1935-2735
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57469
dc.identifier.wosWOS:000382390800010
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos Neglected Tropical Diseases
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleLipophosphoglycans from Leishmania amazonensis Strains Display Immunomodulatory Properties via TLR4 and Do Not Affect Sand Fly Infectionen
dc.typeinfo:eu-repo/semantics/article
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