Experimental selective choriocapillaris photothrombosis using a modified indocyanine green formulation

dc.contributor.authorCardillo, J. A. [UNIFESP]
dc.contributor.authorJorge, R.
dc.contributor.authorCosta, R. A.
dc.contributor.authorNunes, S. M. T.
dc.contributor.authorLavinsky, D. [UNIFESP]
dc.contributor.authorKuppermann, B. D.
dc.contributor.authorTedesco, A. C.
dc.contributor.authorFarah, M. E. [UNIFESP]
dc.contributor.institutionHosp Olhos Araraquara
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniv Calif Irvine
dc.description.abstractBackground: This in vivo study assessed and compared the effectiveness of an aqueous indocyanine green (ICG) formulation (R-ICG) and a lipid ICG formulation (L-ICG) in occluding the rabbit choriocapillaris, and determined the singlet oxygen quantum yields and aggregation properties of both formulations in vitro.Methods: Singlet oxygen production and aggregation were compared. the eye fundus of 30 albino rabbits was irradiated 0-15 min after dye injection using an 810 nm diode laser. Fluorescein angiography and light microscopy were used to evaluate the safety and efficacy of R-ICG and L-ICG.Results: L-ICG decreased the dimerisation constant and the tendency of ICG to form aggregates, and increased the efficiency of ICG in generating singlet oxygen (R-ICG, Phi Delta= 0.120 and L-ICG, Phi Delta= 0.210). Using a 10 mg/kg dose, choriocapillaris occlusion was achieved at a light dose of 35.8 J/cm(2) with L-ICG and 71.6 J/cm(2) with R-ICG with minimal damage to the neurosensory retina.Conclusion: Restrictions to the use of ICG in aqueous solution, low singlet oxygen quantum yields and high aggregation tendency, were overcome with L-ICG. the lower laser irradiance required to obtain choriocapillaris occlusion may suggest that L-ICG is a more potent and selective photosensitiser than R-ICG.en
dc.description.affiliationHosp Olhos Araraquara, Retinal Diagnost & Treatment Div, BR-14802530 Araraquara, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Paulista Sch Med, Dept Ophthalmol, São Paulo, Brazil
dc.description.affiliationState Univ São Paulo USP Ribeirao Preto, Dept Chem, Riberir Preto, Brazil
dc.description.affiliationUniv Calif Irvine, Dept Ophthalmol, Irvine, CA USA
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Paulista Sch Med, Dept Ophthalmol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.identifier.citationBritish Journal of Ophthalmology. London: B M J Publishing Group, v. 92, n. 2, p. 276-280, 2008.
dc.publisherB M J Publishing Group
dc.relation.ispartofBritish Journal of Ophthalmology
dc.rightsAcesso restrito
dc.titleExperimental selective choriocapillaris photothrombosis using a modified indocyanine green formulationen