Antigenotoxicity and antimutagenicity of lycopene in HepG2 cell line evaluated by the comet assay and micronucleus test

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dc.contributor.authorScolastici, Clarissa
dc.contributor.authorLima, Rodrigo Otavio Alves de
dc.contributor.authorBarbisan, Luis Fernando
dc.contributor.authorFerreira, Ana Lucia dos Anjos
dc.contributor.authorRibeiro, Daniel Araki [UNIFESP]
dc.contributor.authorSalvadori, Daisy Maria Favero
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:49:39Z
dc.date.available2016-01-24T13:49:39Z
dc.date.issued2008-03-01
dc.description.abstractEpidemiological studies have provided evidence that high consumption of tomatoes effectively reduces the risk of reactive oxygen species (ROS)-mediated diseases such as cancer. Tomatoes are rich sources of lycopene, a potent singlet oxygen-quenching carotenoid. in addition to its antioxidant properties, lycopene shows an array of biological effects including antimutagenic and anticarcinogenic activities. in the present study, the chemopreventive action of lycopene was examined on DNA damage and clastogenic or aneugenic effects of H2O2 and n-nitrosodiethylamine (DEN) in the metabolically competent human hepatoma cell line (HepG2 cells). Lycopene at concentrations of 10. 25, and 50 mu M, was tested under three protocols: before, simultaneously, and after treatment with the mutagen, using the comet and micronucleus assays. Lycopene significantly reduced the genotoxicity and mutagenicity of H2O2 in all of the conditions tested. for DEN, significant reductions of primary DNA damage (comet assay) were detected when the carotenoid (all of the doses) was added in the cell culture medium before or simultaneously with the mutagen. in the micronucleus test, the protective effect of lycopene was observed only when added prior to DEN treatment. in conclusion, our results suggest that lycopene is a suitable agent for preventing chemically-induced DNA and chromosome damage. (C) 2007 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv São Paulo, UNESP, Botucatu Med Sch, Dept Pathol, BR-05508 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, UNESP, Inst Biosci, Botucatu, SP, Brazil
dc.description.affiliationUniv São Paulo, UNESP, Botucatu Med Sch, Dept Clin Med, BR-05508 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Dept Hlth Sci, Botucatu, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Dept Hlth Sci, Botucatu, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent510-514
dc.identifierhttps://dx.doi.org/10.1016/j.tiv.2007.11.002
dc.identifier.citationToxicology in Vitro. Oxford: Pergamon-Elsevier B.V., v. 22, n. 2, p. 510-514, 2008.
dc.identifier.doi10.1016/j.tiv.2007.11.002
dc.identifier.issn0887-2333
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/30513
dc.identifier.wosWOS:000254694500026
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofToxicology in Vitro
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectLycopeneen
dc.subjectDNA damageen
dc.subjectAntigenotoxicityen
dc.subjectAntimutagenicityen
dc.subjectHepG2 cellsen
dc.titleAntigenotoxicity and antimutagenicity of lycopene in HepG2 cell line evaluated by the comet assay and micronucleus testen
dc.typeinfo:eu-repo/semantics/article
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