Cockroach allergens and asthma in Brazil: Identification of tropomyosin as a major allergen with potential cross-reactivity with mite and shrimp allergens

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dc.contributor.authorSantos, ABR
dc.contributor.authorChapman, M. D.
dc.contributor.authorAalberse, R. C.
dc.contributor.authorVailes, L. D.
dc.contributor.authorFerriani, VPL
dc.contributor.authorOliver, C.
dc.contributor.authorRizzo, M. C.
dc.contributor.authorNaspitz, C. K.
dc.contributor.authorArruda, L. K.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionNetherlands Red Cross
dc.contributor.institutionUniv Virginia
dc.date.accessioned2016-01-24T12:30:52Z
dc.date.available2016-01-24T12:30:52Z
dc.date.issued1999-08-01
dc.description.abstractBackground: Cockroaches produce several proteins that induce IgE antibody responses. Although cockroaches are abundant in warm and humid areas, sensitization to cockroach allergens has not been investigated in Brazil.Objective: the aims of this study were to investigate the frequency of cockroach allergy among patients with asthma, rhinitis, or both in Brazil and to identify American cockroach allergens.Methods: Skin tests using cockroach extracts were performed on children and young adults with asthma, rhinitis, or both. A Periplaneta americana complementary (c)DNA library was screened by using IgE antibodies from Brazilian patients allergic to cockroaches. Reactivity of an mAb directed to Dermatophagoides pteronyssinus tropomyosin against cockroach tissue was examined by immunofluorescence.Results: Cockroach allergy was present in 55% and 79% of the patients, as determined by using skin prick tests alone or combined prick and intradermal tests, respectively. Five cDNA clones reacted with IgE antibody and contained the same sequence. A representative clone (1300 bp), pa12, coded for a protein that reacted with 50% of the sera from patients allergic to cockroaches on plaque immunoassay and showed a high degree of homology to tropomyosins, particularly those from invertebrates. P americana tropomyosin showed 80%, 81%, and 82% sequence identity to tropomyosins from D pteronyssinus, D farinae, and shrimp, respectively, which have been previously defined as important allergens. An mAb directed against D pteronyssinus tropomyosin, which also recognizes shrimp tropomyosin, showed binding to cockroach striated muscle.Conclusion: Our results support the recommendation that cockroach extracts should be routinely used for the evaluation of patients with asthma, rhinitis, or both in Brazil. the identification of P americana tropomyosin as an important allergen Hill make it possible to investigate cross-reactivity among cockroaches, mites, and food derived from invertebrates.en
dc.description.affiliationUSP, Sch Med Ribeirao Preto, Fac Med Ribeirao Preto, Dept Immunol, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUSP, Sch Med Ribeirao Preto, Dept Pediat, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUSP, Sch Med Ribeirao Preto, Dept Morphol, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUNIFESP, Paulista Sch Med, Div Pediat Allergy Clin Immunol & Rheumatol, São Paulo, Brazil
dc.description.affiliationNetherlands Red Cross, Blood Transfus Serv, Cent Labs, Amsterdam, Netherlands
dc.description.affiliationUniv Virginia, Asthma & Allerg Dis Ctr, Charlottesville, VA USA
dc.description.affiliationUnifespUNIFESP, Paulista Sch Med, Div Pediat Allergy Clin Immunol & Rheumatol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent329-337
dc.identifierhttp://dx.doi.org/10.1016/S0091-6749(99)70375-1
dc.identifier.citationJournal of Allergy and Clinical Immunology. St Louis: Mosby-year Book Inc, v. 104, n. 2, p. 329-337, 1999.
dc.identifier.doi10.1016/S0091-6749(99)70375-1
dc.identifier.issn0091-6749
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26125
dc.identifier.wosWOS:000082364800024
dc.language.isoeng
dc.publisherMosby-year Book Inc
dc.relation.ispartofJournal of Allergy and Clinical Immunology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectcockroachen
dc.subjecttropomyosinen
dc.subjectasthmaen
dc.subjectmitesen
dc.subjectshrimpen
dc.subjectallergyen
dc.subjectPeriplaneta americanaen
dc.titleCockroach allergens and asthma in Brazil: Identification of tropomyosin as a major allergen with potential cross-reactivity with mite and shrimp allergensen
dc.typeinfo:eu-repo/semantics/article
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