Differential seminal plasma proteome according to semen retrieval in men with spinal cord injury

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dc.contributor.authorSilva, Barbara Ferreira da [UNIFESP]
dc.contributor.authorSouza, Gustavo Henrique Martins Ferreira
dc.contributor.authorLo Turco, Edson Guimaraes [UNIFESP]
dc.contributor.authorDel Giudice, Paula Toni [UNIFESP]
dc.contributor.authorSoler, Thiesa Butterby [UNIFESP]
dc.contributor.authorSpaine, Deborah Montagnini [UNIFESP]
dc.contributor.authorBorrelli Junior, Milton [UNIFESP]
dc.contributor.authorGozzo, Fabio Cesar
dc.contributor.authorPilau, Eduardo Jorge
dc.contributor.authorGarcia, Jerusa Simone
dc.contributor.authorFerreira, Christina Ramires
dc.contributor.authorEberlin, Marcos Nogueira
dc.contributor.authorBertolla, Ricardo Pimenta [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionWaters Corp
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionNatl Inst Bioanalyt Sci & Technol
dc.contributor.institutionUniversidade Estadual de Maringá (UEM)
dc.contributor.institutionAlfenas Fed Univ
dc.date.accessioned2016-01-24T14:34:27Z
dc.date.available2016-01-24T14:34:27Z
dc.date.issued2013-10-01
dc.description.abstractObjective: To evaluate protein expression profile and to quantify proteins present in seminal plasma from men with spinal cord injury (SCI) and healthy men without SCI.Design: Experimental study.Setting: University hospital.Patient(s): Twelve SCI patients divided into two groups, six who underwent electroejaculation (EEJ) and six who underwent penile vibratory stimulation (PVS); and ten control subjects presenting normal sperm motility and concentration.Intervention(s): EEJ and PVS.Main Outcome Measure(s): the seminal plasma protein profile was analyzed by two proteomic strategies: data-independent label-free quantitative proteomics (MSE) and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (2D SDS-PAGE).Result(s): A total of 638 different proteins were identified by MSE and 18 by 2D SDS-PAGE followed by tandem mass spectrometry. Interactome analysis showed key reproductive biologic processes-insemination, sperm and oocyte fusion, and acrosome reaction-related to all groups, as were triglyceride stimuli. Processes related to actin and muscle function and to iron oxidation, transportation, and homeostasis were found only in the EEJ and PVS groups; response to hydrogen peroxide and increased immune response was found only in the PVS group.Conclusion(s): This study was able to demonstrate differential protein expression among control, PVS, and EEJ groups; SCI is responsible for alterations in seminal plasma protein profile leading to a deviation from homeostasis; proteins reported in both PVS and EEJ groups correlate with the pathophysiology of SCI-related infertility. (Fertil Steril (R) 2013; 100: 959-69. (C) 2013 by American Society for Reproductive Medicine.)en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Surg, Div Urol, Human Reprod Sect, BR-04039060 São Paulo, Brazil
dc.description.affiliationWaters Corp, Mass Spectrometry Applicat Res & Dev Lab, São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Thomson Mass Spectrometry Lab, Campinas, Brazil
dc.description.affiliationUniv Estadual Campinas, Inst Chem, Campinas, Brazil
dc.description.affiliationNatl Inst Bioanalyt Sci & Technol, São Paulo, Brazil
dc.description.affiliationUniv Estadual Maringa, Ctr Exact Sci, Dept Chem, Maringa, Parana, Brazil
dc.description.affiliationAlfenas Fed Univ, Inst Exact Sci, Alfenas, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Surg, Div Urol, Human Reprod Sect, BR-04039060 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent959-+
dc.identifierhttp://dx.doi.org/10.1016/j.fertnstert.2013.06.009
dc.identifier.citationFertility and Sterility. New York: Elsevier B.V., v. 100, n. 4, p. 959-+, 2013.
dc.identifier.doi10.1016/j.fertnstert.2013.06.009
dc.identifier.issn0015-0282
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36772
dc.identifier.wosWOS:000324995200029
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofFertility and Sterility
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectBiomarkersen
dc.subjectspinal cord injuryen
dc.subjectseminal plasmaen
dc.subjectproteomicsen
dc.subjectfunctional enrichmenten
dc.titleDifferential seminal plasma proteome according to semen retrieval in men with spinal cord injuryen
dc.typeinfo:eu-repo/semantics/article
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