Cytotoxicity and genotoxicity of low doses of mercury chloride and methylmercury chloride on human lymphocytes in vitro

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dc.contributor.authorSilva-Pereira, Liz Carmem
dc.contributor.authorCardoso, Plinio Cerqueira dos Santos
dc.contributor.authorLeite, Daniel Silva
dc.contributor.authorBahia, Marcelo de Oliveira
dc.contributor.authorBastos, Wanderley Rodrigues
dc.contributor.authorSmith, Marilia de Arruda Cardoso [UNIFESP]
dc.contributor.authorBurbano, Rommel Rodríguez [UNIFESP]
dc.contributor.institutionFaculdade de Itaituba Departamento de Pós-Graduação
dc.contributor.institutionUniversidade Federal do Pará Centro de Ciências Biológicas Departamento de Biologia
dc.contributor.institutionUniversidade Federal do Pará Centro de Ciências Biológicas Departamento de Patologia
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionFundação Universidade Federal de Rondônia Departamento de Medicina Laboratório de Biogeoquímica Ambiental
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:31:38Z
dc.date.available2015-06-14T13:31:38Z
dc.date.issued2005-06-01
dc.description.abstractMercury is a xenobiotic metal that is a highly deleterious environmental pollutant. The biotransformation of mercury chloride (HgCl2) into methylmercury chloride (CH3HgCl) in aquatic environments is well-known and humans are exposed by consumption of contaminated fish, shellfish and algae. The objective of the present study was to determine the changes induced in vitro by two mercury compounds (HgCl2 and CH3HgCl) in cultured human lymphocytes. Short-term human leukocyte cultures from 10 healthy donors (5 females and 5 males) were set-up by adding drops of whole blood in complete medium. Cultures were separately and simultaneously treated with low doses (0.1 to 1000 µg/l) of HgCl2 and CH3HgCl and incubated at 37ºC for 48 h. Genotoxicity was assessed by chromosome aberrations and polyploid cells. Mitotic index was used as a measure of cytotoxicity. A significant increase (P < 0.05) in the relative frequency of chromosome aberrations was observed for all concentrations of CH3HgCl when compared to control, whether alone or in an evident sinergistic combination with HgCl2. The frequency of polyploid cells was also significantly increased (P < 0.05) when compared to control after exposure to all concentrations of CH3HgCl alone or in combination with HgCl2. CH3HgCl significantly decreased (P < 0.05) the mitotic index at 100 and 1000 µg/l alone, and at 1, 10, 100, and 1000 µg/l when combined with HgCl2, showing a synergistic cytotoxic effect. Our data showed that low concentrations of CH3HgCl might be cytotoxic/genotoxic. Such effects may indicate early cellular changes with possible biological consequences and should be considered in the preliminary evaluation of the risks of populations exposed in vivo to low doses of mercury.en
dc.description.affiliationFaculdade de Itaituba Departamento de Pós-Graduação
dc.description.affiliationUniversidade Federal do Pará Centro de Ciências Biológicas Departamento de Biologia
dc.description.affiliationUniversidade Federal do Pará Centro de Ciências Biológicas Departamento de Patologia
dc.description.affiliationUniversidade de São Paulo Faculdade de Medicina de Ribeirão Preto Departamento de Genética
dc.description.affiliationFundação Universidade Federal de Rondônia Departamento de Medicina Laboratório de Biogeoquímica Ambiental
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Morfologia
dc.description.affiliationUnifespUNIFESP, EPM, Depto. de Morfologia
dc.description.sourceSciELO
dc.format.extent901-907
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2005000600012
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 6, p. 901-907, 2005.
dc.identifier.doi10.1590/S0100-879X2005000600012
dc.identifier.fileS0100-879X2005000600012.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2005000600012
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/2578
dc.identifier.wosWOS:000230125300012
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMethylmercuryen
dc.subjectCytotoxicityen
dc.subjectGenotoxicityen
dc.subjectMitotic indexen
dc.subjectHuman lymphocytesen
dc.subjectChromosome aberrationsen
dc.titleCytotoxicity and genotoxicity of low doses of mercury chloride and methylmercury chloride on human lymphocytes in vitroen
dc.typeinfo:eu-repo/semantics/article
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