Fucan inhibits Chinese hamster ovary cell (CHO) adhesion to fibronectin by binding to the extracellular matrix

Página do item simplificado
dc.contributor.authorRocha, Hugo Alexandre de Oliveira [UNIFESP]
dc.contributor.authorFranco, Celia Regina Cavichiolo
dc.contributor.authorTrindade, Edvaldo da Silva
dc.contributor.authorVeiga, Silvio Sanches
dc.contributor.authorLeite, Edda Lisboa
dc.contributor.authorNader, Helena Bonciani [UNIFESP]
dc.contributor.authorDietrich, Carl Peter [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Fed Rio Grande Norte
dc.contributor.institutionUniv Fed Parana
dc.date.accessioned2016-01-24T12:37:57Z
dc.date.available2016-01-24T12:37:57Z
dc.date.issued2005-07-01
dc.description.abstractIn recent years, sulfated fucans have emerged as an important class of natural biopolymers. in this study, the anti-adhesive activity of a fucan from the brown seaweed Spatoglossum schroederi was analyzed using tumorigenic cells: wild-type Chinese hamster ovary cells (CHO-K1) and the mutant type deficient in xylosyltransferase (CHO-745). Fibronectin (FN) was used as substrate for cell attachment. for both cell types, this fucan has shown a dose-dependent anti-adhesive effect, reaching saturation at around 400 mu g/mL. This effect was abolished by desulfation of the fucan. in addition, this polymer exhibited the highest inhibitory effect in comparison to other sulfated polysaccharides. the fucan was biotinylated and used as a probe to identify its action sites. Biotinylated fucan was detected in the extracellular matrix environment by confocal microscopy and flow cytometric analysis, but not at the cell surface. the results suggest that the fucan shows anti-adhesive activity by binding directly to FN, and blocking FN sites that are recognized by cell surface ligands, possibly the integrin family.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Bioquim, Escola Paulista Med, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniv Fed Rio Grande Norte, Lab Biotecnol Polimeros Nat, BIOPOL, Dept Bioquim, BR-59072970 Natal, RN, Brazil
dc.description.affiliationUniv Fed Parana, Dept Biol Celular, BR-80060000 Curitiba, Parana, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Bioquim, Escola Paulista Med, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent628-633
dc.identifierhttps://dx.doi.org/10.1055/s-2005-871268
dc.identifier.citationPlanta Medica. Stuttgart: Georg Thieme Verlag Kg, v. 71, n. 7, p. 628-633, 2005.
dc.identifier.doi10.1055/s-2005-871268
dc.identifier.issn0032-0943
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/28377
dc.identifier.wosWOS:000231031600008
dc.language.isoeng
dc.publisherGeorg Thieme Verlag Kg
dc.relation.ispartofPlanta Medica
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCHO cellsen
dc.subjectCell adhesionen
dc.subjectAnti-adhesive fucanen
dc.subjectFucoidanen
dc.subjectSpatoglossum schroederien
dc.subjectPhaeophyceaeen
dc.titleFucan inhibits Chinese hamster ovary cell (CHO) adhesion to fibronectin by binding to the extracellular matrixen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
EPM - Artigos