Comparative proteomic analysis reveals that T3SS, Tfp, and xanthan gum are key factors in initial stages of Citrus sinensis infection by Xanthomonas citri subsp citri

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dc.contributor.authorFacincani, Agda P.
dc.contributor.authorMoreira, Leandro M.
dc.contributor.authorSoares, Marcia R.
dc.contributor.authorFerreira, Cristiano B.
dc.contributor.authorFerreira, Rafael M.
dc.contributor.authorFerro, Maria I. T.
dc.contributor.authorFerro, Jesus A.
dc.contributor.authorGozzo, Fabio C.
dc.contributor.authorOliveira, Julio C. F. de [UNIFESP]
dc.contributor.institutionUNESP Univ Estadual Paulista
dc.contributor.institutionUniv Fed Ouro Preto
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:35:22Z
dc.date.available2016-01-24T14:35:22Z
dc.date.issued2014-03-01
dc.description.abstractThe bacteria Xanthomonas citri subsp. citri (Xac) is the causal agent of citrus canker. the disease symptoms are characterized by localized host cell hyperplasia followed by tissue necrosis at the infected area. An arsenal of bacterial pathogenicity- and virulence-related proteins is expressed to ensure a successful infection process. At the post-genomic stage of Xac, we used a proteomic approach to analyze the proteins that are displayed differentially over time when the pathogen attacks the host plant. Protein extracts were prepared from infectious Xac grown in inducing medium (XAM1) for 24 h or from host citrus plants for 3 or 5 days after infection, detached times to evaluate the adaptation and virulence of the pathogen. the protein extracts were proteolyzed, and the peptides derived from tryptic digestion were investigated using liquid chromatography and tandem mass spectrometry. Changes in the protein expression profile were compared with the Xac genome and the proteome recently described under non-infectious conditions. An analysis of the proteome of Xac under infectious conditions revealed proteins directly involved in virulence such as the type III secretion system (T3SS) and effector proteins (T3SS-e), the type IV pilus (Tfp), and xanthan gum biosynthesis. Moreover, four new mutants related to proteins detected in the proteome and with different functions exhibited reduced virulence relative to the wild-type proteins. the results of the proteome analysis of infectious Xac define the processes of adaptation to the host and demonstrate the induction of the virulence factors of Xac involved in plant-pathogen interactions.en
dc.description.affiliationUNESP Univ Estadual Paulista, Dept Tecnol, Fac Ciencias Agr & Vet Jaboticabal, São Paulo, Brazil
dc.description.affiliationUniv Fed Ouro Preto, Dept Ciencias Biol, Inst Ciencias Exatas & Biol, Ouro Preto, MG, Brazil
dc.description.affiliationUniv Fed Ouro Preto, Nucleo Pesquisas Ciencias Biol NUPEB, Ouro Preto, MG, Brazil
dc.description.affiliationUniv Fed Rio de Janeiro UFRJ, Inst Quim, Dept Bioquim, Rio de Janeiro, RJ, Brazil
dc.description.affiliationUniv Estadual Campinas, UNICAMP, Inst Quim, Campinas, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo UNIFESP, Dept Ciencias Biol, Diadema, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo UNIFESP, Dept Ciencias Biol, Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipIDFAPESP: 04/02006-7
dc.description.sponsorshipIDFAPEMIG: FAPEMIG-CBB-APQ-04425-10
dc.format.extent205-217
dc.identifierhttp://dx.doi.org/10.1007/s10142-013-0340-5
dc.identifier.citationFunctional & Integrative Genomics. Heidelberg: Springer Heidelberg, v. 14, n. 1, p. 205-217, 2014.
dc.identifier.doi10.1007/s10142-013-0340-5
dc.identifier.issn1438-793X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/37478
dc.identifier.wosWOS:000333697500017
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofFunctional & Integrative Genomics
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectCitrus cankeren
dc.subjectPlant-pathogen interactionen
dc.subjectXanthan gumen
dc.subjectT3SSen
dc.subjectTfpen
dc.titleComparative proteomic analysis reveals that T3SS, Tfp, and xanthan gum are key factors in initial stages of Citrus sinensis infection by Xanthomonas citri subsp citrien
dc.typeinfo:eu-repo/semantics/article
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