BmTI antigens induce a bovine protective immune response against Boophilus microplus tick

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dc.contributor.authorAndreotti, R.
dc.contributor.authorGomes, A.
dc.contributor.authorMalavazi-Piza, K. C.
dc.contributor.authorSasaki, S. D.
dc.contributor.authorSampaio, CAM
dc.contributor.authorTanaka, A. S.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.date.accessioned2016-01-24T12:33:18Z
dc.date.available2016-01-24T12:33:18Z
dc.date.issued2002-03-01
dc.description.abstractBoophilus microplus trypsin inhibitors (BmTIs) present in larvae were preliminarily characterized as active proteins, approximately 10-18 kDa, by SDS-PAGE. BmTIs showed trypsin inhibitory activity on reverse zymography containing gelatin (0.03%) and also inhibited others serine proteinases (human neutrophil elastase and human plasma kallikrein). Bos indicus, Nelore breed calves, previously sensitized with BmTIs and challenged with tick larvae (20,000 larvae/animal), showed 72.8% efficacy to interfere in tick development with 69.7% and 71.3% reduction of both tick number and egg weight, respectively. Cattle BmTIs antiserum titer was approximately 1:8000. the maximum level of BmTIs antibody production was detected 40 days after the first immunization by ELISA. Our preliminary results suggest that B. microplus serine proteinase inhibitors may play a role in the tick larvae fixation and feeding processes. Therefore, the development of antibodies against BmTIs might impair the normal parasitism. (C) 2002 Elsevier Science B.V. All rights reserved.en
dc.description.affiliationUNIFESP, EPM, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationEmbrapa Gado Corte, BR-79002970 Campo Grande, MS, Brazil
dc.description.affiliationUnifespUNIFESP, EPM, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent557-563
dc.identifierhttp://dx.doi.org/10.1016/S1567-5769(01)00203-X
dc.identifier.citationInternational Immunopharmacology. Amsterdam: Elsevier B.V., v. 2, n. 4, p. 557-563, 2002.
dc.identifier.doi10.1016/S1567-5769(01)00203-X
dc.identifier.issn1567-5769
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26790
dc.identifier.wosWOS:000174814500015
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofInternational Immunopharmacology
dc.rightsAcesso restrito
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectserine proteinase inhibitoren
dc.subjectBoophilus microplusen
dc.subjectticken
dc.subjectbovineen
dc.subjectvaccineen
dc.titleBmTI antigens induce a bovine protective immune response against Boophilus microplus ticken
dc.typeArtigo
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