Determination of eight fatty acid ethyl esters in meconium samples by headspace solid-phase microextraction and gas chromatography-mass spectrometry

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dc.contributor.authorRoehsig, Marli
dc.contributor.authorLouzada de Paula, Daniela Mendes
dc.contributor.authorMoura, Sidnei
dc.contributor.authorAlbuquerque Diniz, Edna Maria de [UNIFESP]
dc.contributor.authorYonamine, Mauricio
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:05:06Z
dc.date.available2016-01-24T14:05:06Z
dc.date.issued2010-07-01
dc.description.abstractA number of fatty acid ethyl esters (FAEEs) have recently been detected in meconium samples. Several of these FAEEs have been evaluated as possible biomarkers for in utero ethanol exposure. in the present study, a method was optimized and validated for the simultaneous determination of eight FAEEs (ethyl laurate, ethyl myristate, ethyl palmitate, ethyl palmitoleate, ethyl stearate, ethyl oleate, ethyl linoleate and ethyl arachidonate) in meconium samples. FAEEs were extracted by headspace solid-phase microextraction. Analyte detection and quantification were carried out using GC-MS operated in chemical ionization mode. the corresponding D5-ethyl esters were synthesized and used as internal standards. the LOQ and LOD for each analyte were <150 and <100 ng/g, respectively. the method showed good linearity (r(2)>0.98) in the concentration range studied (LOQ -2000 ng/g). the intra- and interday imprecision, given by the RSD of the method, was lower than 15% for all FAEEs studied. the validated method was applied to 63 authentic specimens. FAEEs could be detected in alcohol-exposed newborns ( >600 ng/g cumulative concentration). Interestingly, FAEEs could also be detected in some non-exposed newborns, although the concentrations were much lower than those measured in exposed cases.en
dc.description.affiliationUniv São Paulo, Coll Pharmaceut Sci, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniv Hosp São Paulo, Dept Pediat, São Paulo, Brazil
dc.description.affiliationUnifespUniv Hosp São Paulo, Dept Pediat, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2007/00465-2
dc.description.sponsorshipIDFAPESP: 2007/01420-2
dc.format.extent2115-2122
dc.identifierhttp://dx.doi.org/10.1002/jssc.201000118
dc.identifier.citationJournal of Separation Science. Weinheim: Wiley-v C H Verlag Gmbh, v. 33, n. 14, p. 2115-2122, 2010.
dc.identifier.doi10.1002/jssc.201000118
dc.identifier.issn1615-9306
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32662
dc.identifier.wosWOS:000280621900009
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Separation Science
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectFatty acid ethyl esteren
dc.subjectGC-MSen
dc.subjectHeadspace solid-phase microextracionen
dc.subjectMeconiumen
dc.titleDetermination of eight fatty acid ethyl esters in meconium samples by headspace solid-phase microextraction and gas chromatography-mass spectrometryen
dc.typeinfo:eu-repo/semantics/article
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