HeLa-cell adherence patterns and actin aggregation of enteropathogenic Escherichia coli (EPEC) and Shiga-toxin-producing E. coli (STEC) strains carrying different eae and tir alleles

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dc.contributor.authorMora, Azucena [UNIFESP]
dc.contributor.authorBlanco, Miguel
dc.contributor.authorYamamoto, Denise [UNIFESP]
dc.contributor.authorDahbi, Ghizlane
dc.contributor.authorBlanco, Jesus E.
dc.contributor.authorLopez, Cecilia
dc.contributor.authorAlonso, Maria P.
dc.contributor.authorVieira, Monica A. M. [UNIFESP]
dc.contributor.authorHernandes, Rodrigo T. [UNIFESP]
dc.contributor.authorAbe, Cecilia Mari [UNIFESP]
dc.contributor.authorPiazza, Roxane M. F.
dc.contributor.authorLacher, David W.
dc.contributor.authorElias Junior, Waldir Pereira [UNIFESP]
dc.contributor.authorGomes, Tania Aparecida Tardelli [UNIFESP]
dc.contributor.authorBlanco, Jorge
dc.contributor.institutionUniv Santiago de Compostela
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionButantan Inst
dc.contributor.institutionCalde Xeral Hosp
dc.contributor.institutionUS FDA
dc.date.accessioned2016-01-24T13:58:56Z
dc.date.available2016-01-24T13:58:56Z
dc.date.issued2009-12-01
dc.description.abstractA collection of 69 eae-positive strains expressing 29 different intimin types and eight fir alleles was characterized with respect to their adherence patterns to HeLa cells, ability to promote actin accumulation in vitro, the presence of bfpA alleles in positive strains, and bundle-forming pilus (BFP) expression. All of the nine typical enteropathogenic Escherichia coli (tEPEC) studied harbored the enteropathogenic E. coli adherence factor (EAF) plasmid, as shown by PCR and/or EAF probe results. in addition, they were positive for bfpA, as shown by PCR, and BFP expression, as confirmed by immunofluorescence (IFL) and/or immunoblotting (IBL) assays. Localized adherence (LA) was exclusively displayed by those nine tEPEC, while localized-adherence-like (LAL) was the most frequent pattern among atypical EPEC (aEPEC) and Shiga-toxin-producing E. coli (STEC). All LA and LAL strains were able to cause attaching and effacing (AE) lesions, as established by means of the FAS test. There was a significant association between the presence of tir allele alpha 1 and bfpA-positive strains, and consequently, with the LA pattern. However, intimin type or bfpA was not associated with the adherence pattern displayed in HeLa cells. Among the eight bfpA alleles detected, a new type (beta 10; accession number FN391178) was identified in a strain of serotype O157:H45, and a truncated variant (beta 3.2-t; accession number FN 39118 1) in four strains belonging to different pathotypes. [Int Microbiol 2009; 12(4):243-251]en
dc.description.affiliationUniv Santiago de Compostela, Fac Vet Sci, Dept Microbiol & Parasitol, Lugo 27002, Spain
dc.description.affiliationUniversidade Federal de São Paulo, São Paulo Sch Med, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliationButantan Inst, Bacteriol Lab, São Paulo, Brazil
dc.description.affiliationCalde Xeral Hosp, Microbiol Unit, Lugo, Spain
dc.description.affiliationUS FDA, Div Mol Biol, Ctr Food Safety & Appl Nutr, Laurel, MD USA
dc.description.affiliationUnifespUniversidade Federal de São Paulo, São Paulo Sch Med, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipEuropean Commission
dc.description.sponsorshipMinisterio de Sanidad y Consumo de Espana
dc.description.sponsorshipMinisterio de Educacion y Ciencia de Espana
dc.description.sponsorshipXunta de Galicia
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDEuropean Commission: FAIR6-CT-4093
dc.description.sponsorshipIDEuropean Commission: FOOD-CT-2006-36256
dc.description.sponsorshipIDMinisterio de Sanidad y Consumo de Espana: FIS G03-025-COLIRED-O157
dc.description.sponsorshipIDMinisterio de Sanidad y Consumo de Espana: PI052023
dc.description.sponsorshipIDMinisterio de Sanidad y Consumo de Espana: PI051481
dc.description.sponsorshipIDMinisterio de Sanidad y Consumo de Espana: REIPI RD06/0008/1018-1016
dc.description.sponsorshipIDMinisterio de Educacion y Ciencia de Espana: AGL-2008-02129
dc.description.sponsorshipIDXunta de Galicia: PGIDIT 05BTF26101P
dc.description.sponsorshipIDXunta de Galicia: PGIDIT065TAL26101P
dc.description.sponsorshipIDXunta de Galicia: 07MRU036261PR
dc.description.sponsorshipIDXunta de Galicia: 08TAL0172 61PR
dc.description.sponsorshipIDXunta de Galicia: 2007/000044-0
dc.description.sponsorshipIDFAPESP: 08/53812-4
dc.format.extent243-251
dc.identifierhttp://dx.doi.org/10.2436/20.1501.01.104
dc.identifier.citationInternational Microbiology. Barcelona: Viguera Editores, S L, v. 12, n. 4, p. 243-251, 2009.
dc.identifier.doi10.2436/20.1501.01.104
dc.identifier.fileWOS000274010800006.pdf
dc.identifier.issn1139-6709
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31969
dc.identifier.wosWOS:000274010800006
dc.language.isoeng
dc.publisherViguera Editores, S L
dc.relation.ispartofInternational Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectenteropathogenic E. colien
dc.subjectShiga-toxin-producing E. colien
dc.subjectHeLa-cell adherenceen
dc.subjectintiminen
dc.titleHeLa-cell adherence patterns and actin aggregation of enteropathogenic Escherichia coli (EPEC) and Shiga-toxin-producing E. coli (STEC) strains carrying different eae and tir allelesen
dc.typeinfo:eu-repo/semantics/article
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