Effects of low level laser therapy on inflammatory and angiogenic gene expression during the process of bone healing: a microarray analysis

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dc.contributor.authorTim, Carla Roberta
dc.contributor.authorBossini, Paulo Sergio [UNIFESP]
dc.contributor.authorKido, Hueliton Wilian
dc.contributor.authorMalavazi, Iran
dc.contributor.authorvon Zeska Kress, Marcia Regina
dc.contributor.authorCarazzolle, Marcelo Falsarella
dc.contributor.authorParizotto, Nivaldo Antonio
dc.contributor.authorRenno, Ana Claudia Muniz [UNIFESP]
dc.date.accessioned2019-01-21T10:30:13Z
dc.date.available2019-01-21T10:30:13Z
dc.date.issued2016
dc.description.abstractThe process of bone healing as well as the expression of inflammatory and angiogenic genes after low level laser therapy (LLLT) were investigated in an experimental model of bone defects. Sixty Wistar rats were distributed into control group and laser group (830 nm, 30 mW, 2,8 J, 94 seg). Histopathological analysis showed that LLLT was able to modulate the inflammatory process in the area of the bone defect and also to produce an earlier deposition of granulation tissue and newly formed bone tissue. Microarray analysis demonstrated that LLLT produced an up-regulation of the genes related to the inflammatory process (MMD, PTGIR, PTGS2, Ptger2, ILL 1IL6, IL8, IL18) and the angiogenic genes (FGF14, FGF2, ANGPT2, ANGPT4 and PDGFD) at 36 h and 3 days, followed by the decrease of the gene expression on day 7. Immunohistochemical analysis revealed that the subjects that were treated presented a higher expression of COX-2 at 36 h after surgery and an increased VEGF expression on days 3 and 7 after surgery. Our findings indicate that LLLT was efficient on accelerating the development of newly formed bone probably by modulating the inflammatory and angiogenic gene expression as well as COX2 and VEGF immunoexpression during the initial phase of bone healing. (C) 2015 Elsevier B.V. All rights reserved.en
dc.description.affiliationFederal University of São Carlos, Department of Physiotherapy, Rod Washington Luis Km 235, São Carlos 13565-905, Brazil
dc.description.affiliationFederal University of São Paulo, Department of Bioscience, Av. Ana Costa 95, Santos 11050-240, Brazil
dc.description.affiliationFederal University of São Carlos, Department of Genetics and Evolution, Rod Washington Luis Km 235, São Carlos 13565-905, Brazil
dc.description.affiliationUniversity of São Paulo, School of Pharmaceutical Sciences of Ribeirão Preto, Department of Clinical Analysis, Toxicological and Bromatological, Av. do Café 95, Ribeirão Preto, Brazil, 14049-900, Brazil
dc.description.affiliationState University of Campinas, Department of Genetics and Evolution, Cidade Universitária Zeferino Vaz, Campinas 13083-970, Brazil
dc.description.affiliationBrazilian National Center for Research in Energy and Materials, Brazilian Biosciences National Laboratory, Giuseppe Máximo Scolfaro 10.000, Campinas 13083-970, Brazil
dc.description.affiliationUnifespFederal University of São Paulo, Department of Bioscience, Av. Ana Costa 95, Santos 11050-240, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCenter for Computational Engineering and Sciences at UNICAMP SP Brazil (FAPESP/CEPID)
dc.description.sponsorshipIDFAPESP: 2010/15335-0
dc.description.sponsorshipIDFAPESP/CEPID: 2013/08293-7
dc.format.extent8-15
dc.identifierhttps://doi.org/10.1016/j.jphotobiol.2015.10.028
dc.identifier.citationJournal Of Photochemistry And Photobiology B-Biology. Lausanne, v. 154, p. 8-15, 2016.
dc.identifier.doi10.1016/j.jphotobiol.2015.10.028
dc.identifier.issn1011-1344
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/49644
dc.identifier.wosWOS:000368954300002
dc.language.isoeng
dc.publisherElsevier science sa
dc.relation.ispartofJournal Of Photochemistry And Photobiology B-Biology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectBone Repairen
dc.subjectLllten
dc.subjectMicroarrayen
dc.subjectGene ExpressionCell-Proliferationen
dc.subject808 Nmen
dc.subjectRepairen
dc.subjectRatsen
dc.subjectIrradiationen
dc.subjectPhotobiomodulationen
dc.subjectDifferentiationen
dc.subjectRadiationen
dc.subjectCytokinesen
dc.subjectImprovesen
dc.titleEffects of low level laser therapy on inflammatory and angiogenic gene expression during the process of bone healing: a microarray analysisen
dc.typeinfo:eu-repo/semantics/article
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