Representational difference analysis between Afa/Dr diffusely adhering Escherichia coli and nonpathogenic E-coli K-12

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dc.contributor.authorBlanc-Potard, Anne Beatrice
dc.contributor.authorTinsley, Colin
dc.contributor.authorScaletsky, Isabel [UNIFESP]
dc.contributor.authorLe Bouguenec, Chantal
dc.contributor.authorGuignot, Julie
dc.contributor.authorServin, Alain L.
dc.contributor.authorNassif, Xavier
dc.contributor.authorBernet-Camard, Marie Francoise
dc.contributor.institutionFac Pharm Paris 11
dc.contributor.institutionFac Med Necker Enfants Malad
dc.contributor.institutionInst Pasteur
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:33:31Z
dc.date.available2016-01-24T12:33:31Z
dc.date.issued2002-10-01
dc.description.abstractDiffusely adhering Escherichia coli strains harboring Afa/Dr adhesins (Afa/Dr DAEC) have been associated with diarrhea and urinary tract infections (UTIs). the present work is the first extensive molecular study of a Afa/Dr DAEC strain using the representational difference analysis technique. We have searched for DNA sequences present in strain C1845, recovered from a diarrheagenic child, but absent from a nonpathogenic K-12 strain. Strain C1845 harbors part of a pathogenicity island (PAI(CFT073)) and several iron transport systems found in other E. coli pathovars. We did not find genes encoding factors known to subvert host cell proteins, such as type III secretion system or effector proteins. Several C1845-specific sequences are homologous to putative virulence genes or show no homology with known sequences, and we have analyzed their distribution among Afa/Dr and non-Afa/Dr clinical isolates and among strains from the E. coli Reference Collection. Three C1845-specific sequences (MO30, S109, and S111) have a high prevalence (77 to 80%) among Afa/Dr strains and a low prevalence (12 to 23%) among non-Afa/Dr strains. in addition, our results indicate that strain 1H11128, an Afa/Dr DAEC strain recovered from a patient with a UTI, is genetically closely related to strain C1845.en
dc.description.affiliationFac Pharm Paris 11, UFR Pharm Paris 11, INSERM, U510, F-92296 Chatenay Malabry, France
dc.description.affiliationFac Med Necker Enfants Malad, INSERM, U411, F-75730 Paris, France
dc.description.affiliationInst Pasteur, Unite Pathogenie Bacterienne Muqueuses, F-75724 Paris, France
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent5503-5511
dc.identifierhttp://dx.doi.org/10.1128/IAI.70.10.5503-5511.2002
dc.identifier.citationInfection and Immunity. Washington: Amer Soc Microbiology, v. 70, n. 10, p. 5503-5511, 2002.
dc.identifier.doi10.1128/IAI.70.10.5503-5511.2002
dc.identifier.fileWOS000178125500020.pdf
dc.identifier.issn0019-9567
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26985
dc.identifier.wosWOS:000178125500020
dc.language.isoeng
dc.publisherAmer Soc Microbiology
dc.relation.ispartofInfection and Immunity
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleRepresentational difference analysis between Afa/Dr diffusely adhering Escherichia coli and nonpathogenic E-coli K-12en
dc.typeinfo:eu-repo/semantics/article
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