Is Malassezia nana the main species in horses' ear canal microbiome?

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dc.citation.issue3
dc.citation.volume47
dc.contributor.authorAldrovandi, Ana Lucia
dc.contributor.authorOsugui, Lika [UNIFESP]
dc.contributor.authorAcqua Coutinho, Selene Dall'
dc.coverageSao Paulo
dc.date.accessioned2020-08-14T13:44:24Z
dc.date.available2020-08-14T13:44:24Z
dc.date.issued2016
dc.description.abstractThe objective of this study was to characterize genotypically Malassezia spp. isolated from the external ear canal of healthy horses. Fifty-five horses, 39 (70.9%) males and 16 (29.1%) females, from different breeds and adults were studied. External ear canals were cleaned and a sterile cotton swab was introduced to collect cerumen. A total of 110 samples were cultured into Dixon medium and were incubated at 32 degrees C for up to 15 days. Macro- and micromorphology and phenotypic identification were performed. DNA was extracted, strains were submitted to polymerase chain reaction technique, and the products obtained were submitted to Restriction Fragment Length Polymorphism using the restriction enzymes BstCI and HhaI. Strains were sent off to genetic sequencing of the regions 26S rDNA D1/D2 and ITS1-5.85-ITS2 rDNA. Malassezia spp. were isolated from 33/55 (60%) animals and 52/110 (47%) ear canals. No growth on Sabouraud dextrose agar was observed, confirming the lipid dependence of all strains. Polymerase chain reaction-Restriction fragment length polymorphism permitted the molecular identification of Malassezia nana-42/52 (81%) and Malassezia slooffiae- 10/52 (19%). Sequencing confirmed RFLP identification. It was surprising that M. nana represented over 80% of the strains and no Malassezia equina was isolated in this study, differing from what was expected. (C) 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. This is an open access article under the CC BY-NC-ND licenseen
dc.description.affiliationUniv Paulista, Mol & Cellular Biol Lab, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCapes-PROSUP fellowship, Federal Government, Brazil
dc.format.extent770-774
dc.identifierhttp://dx.doi.org/10.1016/j.bjm.2016.04.017
dc.identifier.citationBrazilian Journal Of Microbiology. Sao Paulo, v. 47, n. 3, p. 770-774, 2016.
dc.identifier.doi10.1016/j.bjm.2016.04.017
dc.identifier.fileWOS000381101200033.pdf
dc.identifier.issn1517-8382
dc.identifier.scieloS1517-83822016000300770
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57648
dc.identifier.wosWOS:000381101200033
dc.language.isoeng
dc.publisherSoc Brasileira Microbiologia
dc.relation.ispartofBrazilian Journal Of Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMalassezia nanaen
dc.subjectMalassezia slooffiaeen
dc.subjectMicrobiomeen
dc.subjectHorsesen
dc.subjectEar canalen
dc.titleIs Malassezia nana the main species in horses' ear canal microbiome?en
dc.typeinfo:eu-repo/semantics/article
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