GABAergic pump cells of solitary tract nucleus innervate retrotrapezoid nucleus chemoreceptors

Página do item simplificado
dc.contributor.authorTakakura, Ana C. [UNIFESP]
dc.contributor.authorMoreira, Thiago S. [UNIFESP]
dc.contributor.authorWest, Gavin H.
dc.contributor.authorGwilt, Justin M.
dc.contributor.authorColombari, Eduardo [UNIFESP]
dc.contributor.authorStornetta, Ruth L.
dc.contributor.authorGuyenet, Patrice G.
dc.contributor.institutionUniv Virginia Hlth Syst
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:48:49Z
dc.date.available2016-01-24T13:48:49Z
dc.date.issued2007-07-01
dc.description.abstractGABAergic pump cells of solitary tract nucleus innervate retrotrapezoid nucleus chemoreceptors. J Neurophysiol 98: 374 - 381, 2007. First published April 25, 2007; doi: 10.1152/ jn. 00322.2007. the retrotrapezoid nucleus (RTN) contains central respiratory chemoreceptors that are inhibited by activation of slowly adapting pulmonary stretch receptors (SARs). Here we examine whether RTN inhibition by lung inflation could be mediated by a direct projection from SAR second- order neurons (pump cells). Pump cells ( n = 56 neurons, 13 rats) were recorded in the nucleus of solitary tract (NTS) of halothane- anesthetized rats with intact vagus nerves. Pump cells had discharges that coincided with lung inflation as monitored by the tracheal pressure. Their activity increased when end- expiratory pressure was raised and stopped instantly when ventilation was interrupted in expiration. Many pump cells could be antidromically activated from RTN (12/36). Nine of those were labeled with biotinamide. of these nine cells, eight contained glutamic acid decarboxylase 67 (GAD67) mRNA and seven were found to reside in the lower half of the interstitial subnucleus of NTS (iNTS). Using the retrograde tracer cholera toxin-B, we confirmed that neurons located in or close to iNTS innervate RTN (two rats). Many such neurons contained GAD67 mRNA and a few contained glycine transporter2 (GLYT2) mRNA. Anterograde tract tracing with biotinylated dextranamide (four rats) applied to iNTS also confirmed that this region innervates RTN by a predominantly GABAergic projection. This work confirms that many rat NTS pump cells are located in and around the interstitial subnucleus at area postrema level. We demonstrate that a GABAergic subset of these pump cells innervates the RTN region. We conclude that these inhibitory neurons probably contact RTN chemoreceptors and mediate their inhibition by lung inflation.en
dc.description.affiliationUniv Virginia Hlth Syst, Dept Pharmacol, Charlottesville, VA 22908 USA
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Physiol, São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Physiol, São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent374-381
dc.identifierhttp://dx.doi.org/10.1152/jn.00322.2007
dc.identifier.citationJournal of Neurophysiology. Bethesda: Amer Physiological Soc, v. 98, n. 1, p. 374-381, 2007.
dc.identifier.doi10.1152/jn.00322.2007
dc.identifier.issn0022-3077
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/29835
dc.identifier.wosWOS:000247938400036
dc.language.isoeng
dc.publisherAmer Physiological Soc
dc.relation.ispartofJournal of Neurophysiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleGABAergic pump cells of solitary tract nucleus innervate retrotrapezoid nucleus chemoreceptorsen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
Em verificação - Geral