The effects of conjugated estrogen, raloxifene and soy extract on collagen in rat bones

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dc.contributor.authorCondi, F. L. F.
dc.contributor.authorSoares, J. M.
dc.contributor.authorTeodoro, W. R.
dc.contributor.authorVeloso, A. P.
dc.contributor.authorParra, E. R.
dc.contributor.authorJesus Simoes, M. de [UNIFESP]
dc.contributor.authorBaracat, E. C.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:27:52Z
dc.date.available2016-01-24T14:27:52Z
dc.date.issued2012-10-01
dc.description.abstractObjective To evaluate the action of conjugated equine estrogen, raloxifene and isolated or combined genistein-rich soy extracts on collagen fibers in the bones of oophorectomized rats.Materials and methods Seventy female rats received testosterone propionate (0.1 mu g/g) on the 9th day after birth. At 6 months of age, the rats were administered the vehicle (propylene glycol, 0.5 ml/day), and ten of the rats were randomly chosen to comprise the non-oophorectomized control group (GI). the other 60 rats were ovariectomized and randomized into six groups of ten as follows: GII, vehicle; GIII, conjugated equine estrogen (CEE), 50 mu g/kg/day; GIV, raloxifene (RAL), 0.75 mg/kg/day; GV, genistein-rich soy extract (GSE), 300 mg/kg/day; GVI, CEE + GSE, 50 mu g/kg/day + 300 mg/kg/day; and GVII, CEE + RAL, 50 mu g/kg/day + 0.75 mg/kg/day. Three months after surgery, the drugs were administered for 60 consecutive days. All rats were euthanized, and their left tibiae were removed for histological routine. the histological sections were stained with hematoxylin-eosin, and picrosirius for evaluating bone microarchitecture. Types I and II collagen fibers were analyzed by immunofluorescence. Data analysis was carried out with ANOVA and Tukey's test.Results Collagen reduction was significant in the GIII animals when compared to the other groups (p < 0.05). There was no significant difference in the thickness of collagen fibers among the groups. There was a greater quantity of type III collagen in GVI than in the other groups.Conclusion Our data indicate that conjugated equine estrogen improves bone quality because it increases the quantity of type I collagen while reducing the quantity of thin collagen fibers. in addition, the combination of CEE and raloxifene or genistein-rich soy extract is not as efficient as CEE itself to improve bone quality.en
dc.description.affiliationUniv São Paulo, Fac Med, Dept Gynecol, LIM 58, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med, Dept Rheumatol, LIM 17, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Sch Med, Fac Med, Dept Pathol,LIM 5, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Histol & Cellular Biol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Histol & Cellular Biol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent441-448
dc.identifierhttp://dx.doi.org/10.3109/13697137.2011.624213
dc.identifier.citationClimacteric. London: Informa Healthcare, v. 15, n. 5, p. 441-448, 2012.
dc.identifier.doi10.3109/13697137.2011.624213
dc.identifier.issn1369-7137
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35401
dc.identifier.wosWOS:000308942500006
dc.language.isoeng
dc.publisherInforma Healthcare
dc.relation.ispartofClimacteric
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://informahealthcare.com/userimages/ContentEditor/1255620309227/Copyright_And_Permissions.pdf
dc.subjectCONJUGATED EQUINE ESTROGENen
dc.subjectRALOXIFENEen
dc.subjectGENISTEIN-RICH SOY EXTRACTen
dc.subjectBONEen
dc.subjectFEMALE RATSen
dc.titleThe effects of conjugated estrogen, raloxifene and soy extract on collagen in rat bonesen
dc.typeinfo:eu-repo/semantics/article
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