Highly purified glycosylphosphatidylinositols from Trypanosoma cruzi are potent proinflammatory agents

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dc.contributor.authorAlmeida, I. C.
dc.contributor.authorCamargo, M. M.
dc.contributor.authorProcopio, D. O.
dc.contributor.authorSilva, Luiz S. [UNIFESP]
dc.contributor.authorMehlert, A.
dc.contributor.authorTravassos, Luiz Rodolpho [UNIFESP]
dc.contributor.authorGazzinelli, R. T.
dc.contributor.authorFerguson, MAJ
dc.contributor.institutionUniv Dundee
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFundacao Oswaldo Cruz
dc.date.accessioned2016-01-24T12:31:03Z
dc.date.available2016-01-24T12:31:03Z
dc.date.issued2000-04-03
dc.description.abstractIntracellular protozoan parasites are potent stimulators of cell-mediated immunity. the induction of macrophage proinflammatory cytokines by Trypanosoma cruzi is considered to be important in controlling the infection and the outcome of Chagas' disease. Here we show that the potent tumour necrosis factor-alpha-, interleukin-12- and nitric oxide-inducing activities of T.cruzi trypomastigote mucins were recovered quantitatively in a highly purified and characterized glycosylphosphatidylinositol (GPI) anchor fraction of this material. the bioactive trypomastigote GPI fraction was compared with a relatively inactive GPI fraction prepared from T.cruzi epimastigote mucins, the trypomastigote GPI structures were found to contain additional galactose residues and unsaturated, instead of saturated, fatty acids in the sn-2 position of the alkyl-acylglycerolipid component. the latter feature is essential for the extreme potency of the trypomastigote GPI fraction, which is at least as active as bacterial endotoxin and Mycoplasma lipopeptide and, therefore, one of the most potent microbial proinflammatory agents known.en
dc.description.affiliationUniv Dundee, Dept Biochem, Div Mol Parasitol & Biol Chem, Dundee DD1 5EH, Scotland
dc.description.affiliationUniv Fed Minas Gerais, Dept Biochem & Immunol, Belo Horizonte, MG, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Discipline Cell Biol, BR-0423062 São Paulo, Brazil
dc.description.affiliationFundacao Oswaldo Cruz, Ctr Pesquisas Rene Rachou, BR-30190002 Belo Horizonte, MG, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Discipline Cell Biol, BR-0423062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent1476-1485
dc.identifierhttp://dx.doi.org/10.1093/emboj/19.7.1476
dc.identifier.citationEmbo Journal. Oxford: Oxford Univ Press, v. 19, n. 7, p. 1476-1485, 2000.
dc.identifier.doi10.1093/emboj/19.7.1476
dc.identifier.issn0261-4189
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26290
dc.identifier.wosWOS:000086388700008
dc.language.isoeng
dc.publisherOxford Univ Press
dc.relation.ispartofEmbo Journal
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.subjectChagas' diseaseen
dc.subjectcytokinesen
dc.subjectglycosylphosphatidylinositolen
dc.subjectinflammationen
dc.subjectnitric oxideen
dc.titleHighly purified glycosylphosphatidylinositols from Trypanosoma cruzi are potent proinflammatory agentsen
dc.typeinfo:eu-repo/semantics/article
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