Low-level laser therapy enhances the expression of osteogenic factors during bone repair in rats

Página do item simplificado
dc.contributor.authorTim, Carla Roberta
dc.contributor.authorPinto, Karina Nogueira Zambone
dc.contributor.authorRossi, Bruno Rafael Orsini
dc.contributor.authorFernandes, Kelly Rossetti [UNIFESP]
dc.contributor.authorMatsumoto, Mariza Akemi
dc.contributor.authorParizotto, Nivaldo Antonio
dc.contributor.authorRenno, Ana Claudia Muniz [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Sagrado Coracao
dc.date.accessioned2016-01-24T14:35:02Z
dc.date.available2016-01-24T14:35:02Z
dc.date.issued2014-01-01
dc.description.abstractThe aim of this study was to evaluate the effects of low-level laser therapy (LLLT) on bone formation, immunoexpression of osteogenic factors, and biomechanical properties in a tibial bone defect model in rats. Sixty male Wistar rats were distributed into bone defect control group (CG) and laser irradiated group (LG). Animals were euthanized on days 15, 30, and 45 post-injury. the histological and morphometric analysis showed that the treated animals presented no inflammatory infiltrate and a better tissue organization at 15 and 30 days postsurgery. Also, a higher amount of newly formed bone was observed at 15 days postsurgery. No statistically significant difference was observed in cyclooxygenase-2 immunoexpression among the groups at 15, 30, and 45 days in the immunohistochemical analysis. Considering RUNX-2, the immunoexpression was statistically higher in the LG compared to the CG at 45 days. BMP-9 immunoexpression was significantly higher in the LG in comparison to CG at day 30. However, there was no expressivity for this immunomarker, both in the CG and LG, at the day 45 postsurgery. No statistically significant difference was observed in the receptor activator of nuclear factor kappa-B ligand immunoexpression among the groups in all periods evaluated. No statistically significant difference among the groups was observed in the maximal load in any period of time. Our findings indicate that laser therapy improved bone healing by accelerating the development of newly formed bone and activating the osteogenic factors on tibial defects, but the biomechanical properties in LG were not improved.en
dc.description.affiliationUniv Fed Sao Carlos, Dept Physiotherapy, São Paulo, Brazil
dc.description.affiliationUniv Fed Sao Carlos, Dept Morphol & Pathol, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Biomech & Rehabil, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biosci, BR-11050240 São Paulo, Brazil
dc.description.affiliationUniv Sagrado Coracao, Sch Dent, Dept Oral Maxillofacial Surg, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biosci, BR-11050240 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent147-156
dc.identifierhttps://dx.doi.org/10.1007/s10103-013-1302-9
dc.identifier.citationLasers in Medical Science. London: Springer London Ltd, v. 29, n. 1, p. 147-156, 2014.
dc.identifier.doi10.1007/s10103-013-1302-9
dc.identifier.issn0268-8921
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/37229
dc.identifier.wosWOS:000329834200020
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofLasers in Medical Science
dc.rightsAcesso restrito
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectBone repairen
dc.subjectBone defecten
dc.subjectLow-level laser therapyen
dc.subjectLLLTen
dc.titleLow-level laser therapy enhances the expression of osteogenic factors during bone repair in ratsen
dc.typeArtigo
Arquivos
Coleções
ISS - Artigos