Lateral hypothalamic serotonin is not stimulated during central leptin hypophagia

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dc.contributor.authorTelles, Monica Marques [UNIFESP]
dc.contributor.authorSilva, Thais Girão da [UNIFESP]
dc.contributor.authorWatanabe, Regina Lucia Harumi [UNIFESP]
dc.contributor.authorAndrade, Iracema Senna de [UNIFESP]
dc.contributor.authorEstadella, Debora [UNIFESP]
dc.contributor.authorNascimento, Claudia Maria da Penha Oller do [UNIFESP]
dc.contributor.authorOyama, Lila Missae [UNIFESP]
dc.contributor.authorRibeiro, Eliane Beraldi [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:31:53Z
dc.date.available2016-01-24T14:31:53Z
dc.date.issued2013-06-10
dc.description.abstractWhether leptin targets the hypothalamic serotonergic system to inhibit food intake is not established. We examined the effect of a short-term i.c.v. leptin treatment on serotonin microdialysate levels in rat lateral hypothalamus. Adipose tissue gene expression was also evaluated.Male rats received four daily injections of leptin (5 mu g) or vehicle (with pair-feeding to leptin-induced intake) and a fifth injection during collection of LH microdialysates. We found that serotonin and 5-HIAA levels were not affected by the leptin pre-treatment, as basal levels were similar between the leptin and the pair-fed group. These levels remained unaltered after the acute leptin injection.For gene expression studies, rats were pre-treated with five daily injections of either leptin (5 mu g) or vehicle (with either pair-feeding or ad libitum intake). mRNA levels of resistin, adiponectin, lipoprotein lipase, and PPAR-gamma were unaltered by either leptin or pair-feeding. Leptin gene expression was significantly reduced by leptin but not by pair-feeding, in both the retroperitoneal (- 74%) and the epididymal (- 99%) depots while no differences were observed in the subcutaneous depot.The observations confirmed the absence of an acute stimulatory effect of central leptin on serotonin release in the lateral hypothalamus and showed that the pre-treatment with leptin failed to modify this pattern. This indicates that components of the serotonergic system are probably not directly affected by leptin. Additionally, the central effect of leptin was able to downregulate its own adipose tissue gene expression in a depot-specific manner while other adipokine genes were not affected. (C) 2013 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Dept Fisiol, Disciplina Fisiol Nutr, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Ciencias Biol, Diadema, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Dept Fisiol, Disciplina Fisiol Nutr, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Ciencias Biol, Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent75-80
dc.identifierhttps://dx.doi.org/10.1016/j.regpep.2013.03.027
dc.identifier.citationRegulatory Peptides. Amsterdam: Elsevier B.V., v. 184, p. 75-80, 2013.
dc.identifier.doi10.1016/j.regpep.2013.03.027
dc.identifier.fileWOS000320220700011.pdf
dc.identifier.issn0167-0115
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/36424
dc.identifier.wosWOS:000320220700011
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofRegulatory Peptides
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectAdipokinesen
dc.subjectFood intakeen
dc.subjectIn vivo microdialysisen
dc.subjectLeptinen
dc.subjectSerotoninen
dc.titleLateral hypothalamic serotonin is not stimulated during central leptin hypophagiaen
dc.typeinfo:eu-repo/semantics/article
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