Estudo da interação de albumina de soro bovino (BSA) em adsorventes de quitosana/alginato epoxilados: avaliação das isotermas de adsorção e parâmetros termodinâmicos
Data
2018-11-29
Autores
Oliveira, Samanta Maurilio de 
Orientadores
Bresolin, Igor Tadeu Lazzarotto
Tipo
Trabalho de conclusão de curso
Título da Revista
ISSN da Revista
Título de Volume
Resumo
s proteínas são bioprodutos resultantes da indústria biotecnológica que se destacam por suas propriedades enzimáticas e terapêuticas. A albumina, proteína com grande valor comercial, vem sendo amplamente utilizada e um dos grandes desafios é aperfeiçoar os processos de isolamento desse composto buscando um grau de pureza alto e o menor custo possível. Uma das competências da albumina é sua tendência de ligação reversível com diversos ligantes. Existem diversos fatores que afetam essa interação, como, por exemplo, forca iônica, viscosidade, pH e temperatura. Este trabalho teve como objetivo estudar a interação da albumina de soro bovino (BSA) em adsorventes de quitosana/alginato epoxilados, buscando-se avaliar o comportamento em diferentes temperaturas e pHs. Para a avaliação dos parâmetros de adsorção e termodinâmicos, nos experimentos com corantes a adsorção foi estudada nas temperaturas de 25 e 35°C utilizando o tampão biológico fosfato de sódio e MOPS.
Proteins are bioproducts resulting from the biotechnology industry that stand out for their enzymatic and therapeutic properties. Albumin, a protein with great commercial value, has been widely used and one of the great challenges is to improve the isolation processes of this compound, seeking a high degree of purity and the lowest cost. One of its competencies is the tendency to bind reversibly with various binders. There are several factors that affect this interaction, such as ionic strength, viscosity, pH and temperature. The objective of this study was to study the interaction of bovine serum albumin (BSA) in epoxylated chitosan/alginate adsorbents, aiming to evaluate the behavior at different temperatures and pHs. To evaluate the adsorption and thermodynamic parameters, in adsorption experiments the adsorption was studied at temperatures of 25 and 35° C using the biological phosphate buffer and MOPS.
Proteins are bioproducts resulting from the biotechnology industry that stand out for their enzymatic and therapeutic properties. Albumin, a protein with great commercial value, has been widely used and one of the great challenges is to improve the isolation processes of this compound, seeking a high degree of purity and the lowest cost. One of its competencies is the tendency to bind reversibly with various binders. There are several factors that affect this interaction, such as ionic strength, viscosity, pH and temperature. The objective of this study was to study the interaction of bovine serum albumin (BSA) in epoxylated chitosan/alginate adsorbents, aiming to evaluate the behavior at different temperatures and pHs. To evaluate the adsorption and thermodynamic parameters, in adsorption experiments the adsorption was studied at temperatures of 25 and 35° C using the biological phosphate buffer and MOPS.