Interactions of Escherichia coli strains of non-EPEC serogroups that carry eae and lack the EAF and stx gene sequences with undifferentiated and differentiated intestinal human Caco-2 cells

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dc.contributor.authorRosa, Ana Claudia de Paula
dc.contributor.authorVieira, Monica Aparecida Midolli [UNIFESP]
dc.contributor.authorTibana, Anita
dc.contributor.authorGomes, Tania Aparecida Tardelli [UNIFESP]
dc.contributor.authorAndrade, João Ramos da Costa
dc.contributor.institutionUniversidade do Estado do Rio de Janeiro (UERJ)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.date.accessioned2016-01-24T12:31:25Z
dc.date.available2016-01-24T12:31:25Z
dc.date.issued2001-06-12
dc.description.abstractEscherichia coli strains of non-EPEC serotypes that carry eae and lack the EAF and the Shiga toxin (sts) gene sequences have been found in acute diarrhea. Both the cell association and the cell entry of these strains in human intestinal epithelial cells were studied as a function of cell differentiation and polarization. the eae+/EAF-/stx- non-EPEC E. coli strains invaded undifferentiated Caco-2 cells more efficiently than differentiated cells. in contrast, prototype EPEC strain E2348/69 did not show significative differences from invasion rates of undifferentiated and differentiated cells. the uptake of these strains was greatly enhanced by pretreatment of differentiated Caco-2 cells with EGTA. These results suggest that the eae+/EAF-/stx-non-EPEC E. coli invasion of intestinal cells may be dependent on receptors expressed on the surface of undifferentiated cells and the basolateral pole of differentiated cells. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.en
dc.description.affiliationUniv Estado Rio de Janeiro, Fac Ciencias Med, Dept Microbiol & Imunol, BR-20551030 Rio de Janeiro, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniv Fed Rio de Janeiro, Ctr Ciencias Saude, Inst Microbiol, BR-21941590 Rio de Janeiro, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent117-122
dc.identifierhttps://dx.doi.org/10.1111/j.1574-6968.2001.tb10702.x
dc.identifier.citationFems Microbiology Letters. Amsterdam: Elsevier B.V., v. 200, n. 1, p. 117-122, 2001.
dc.identifier.doi10.1111/j.1574-6968.2001.tb10702.x
dc.identifier.issn0378-1097
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/26582
dc.identifier.wosWOS:000169419900019
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofFems Microbiology Letters
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectCaco-2 cellen
dc.subjectEnterocyte differentiationen
dc.subjectBacterial adherenceen
dc.subjectBacterial invasionen
dc.subjectEae plus /EAF-/stx Escherichia colien
dc.titleInteractions of Escherichia coli strains of non-EPEC serogroups that carry eae and lack the EAF and stx gene sequences with undifferentiated and differentiated intestinal human Caco-2 cellsen
dc.typeinfo:eu-repo/semantics/article
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