Towards Understanding Male Infertility After Spinal Cord Injury Using Quantitative Proteomics

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dc.citation.issue4
dc.citation.volume15
dc.contributor.authorSilva, Barbara Ferreira da [UNIFESP]
dc.contributor.authorMeng, Chen
dc.contributor.authorHelm, Dominic
dc.contributor.authorPachl, Fiona
dc.contributor.authorSchiller, Jurgen
dc.contributor.authorIbrahim, Emad
dc.contributor.authorLynne, Charles M.
dc.contributor.authorBrackett, Nancy L.
dc.contributor.authorBertolla, Ricardo Pimenta [UNIFESP]
dc.contributor.authorKuster, Bernhard
dc.coverageBethesda
dc.date.accessioned2020-07-22T13:23:16Z
dc.date.available2020-07-22T13:23:16Z
dc.date.issued2016
dc.description.abstractThe study of male infertility after spinal cord injury (SCI) has enhanced the understanding of seminal plasma (SP) as an important regulator of spermatozoa function. However, the most important factors leading to the diminished sperm motility and viability observed in semen of men with SCI remained unknown. Thus, to explore SP related molecular mechanisms underlying infertility after SCI, we used mass spectrometry-based quantitative proteomics to compare SP retrieved from SCI patients to normal controls. As a result, we present an in-depth characterization of the human SP proteome, identifying approximate to 2,800 individual proteins, and describe, in detail, the differential proteome observed in SCI. Our analysis demonstrates that a hyper-activation of the immune system may influence some seminal processes, which likely are not triggered by microbial infection. Moreover, we show evidence of an important prostate gland functional failure, i.e. diminished abundance of metabolic enzymes related to ATP turnover and those secreted via prostasomes. Further we identify the main outcome related to this fact and that it is intrinsically linked to the low sperm motility in SCI. Together, our data highlights the molecular pathways hindering fertility in SCI and shed new light on other causes of male infertility.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Surg, Div Urol, Human Reprod Sect, Sao Paulo, Brazil
dc.description.affiliationTech Univ Munich, Chair Prote & Bioanalyt, Emil Erlenmeyer Forum 5, D-85354 Freising Weihenstephan, Germany
dc.description.affiliationUniv Leipzig, Dept Med, Inst Med Phys, D-04109 Leipzig, Germany
dc.description.affiliationUniv Miami, Miller Sch Med, Miami Project Cure Paralysis, Miami, FL 33136 USA
dc.description.affiliationUniv Miami, Miller Sch Med, Dept Urol, Miami, FL 33136 USA
dc.description.affiliationTech Univ Munich, Ctr Integrated Prot Sci Munich CIPSM, Freising Weihenstephan, Germany
dc.description.affiliationTech Univ Munich, Bavarian Biomol Mass Spectrometry Ctr, Freising Weihenstephan, Germany
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Surg, Div Urol, Human Reprod Sect, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipGerman Academic Exchange Service (DAAD)
dc.description.sponsorshipBrazilian National Council for Scientific and Technological Development (CNPq)
dc.description.sponsorshipIDDAAD: A/11/71497
dc.format.extent1424-1434
dc.identifierhttp://dx.doi.org/10.1074/mcp.M115.052175
dc.identifier.citationMolecular & Cellular Proteomics. Bethesda, v. 15, n. 4, p. 1424-1434, 2016.
dc.identifier.doi10.1074/mcp.M115.052175
dc.identifier.issn1535-9476
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56130
dc.identifier.wosWOS:000373992600018
dc.language.isoeng
dc.publisherAmer Soc Biochemistry Molecular Biology Inc
dc.relation.ispartofMolecular & Cellular Proteomics
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleTowards Understanding Male Infertility After Spinal Cord Injury Using Quantitative Proteomicsen
dc.typeinfo:eu-repo/semantics/article
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