The extrinsic apoptotic signaling pathway in gastric adenocarcinomas assessed by tissue microarray

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dc.contributor.authorGomes, Thiago Simao [UNIFESP]
dc.contributor.authorOshima, Celina Tizuko Fujiyama [UNIFESP]
dc.contributor.authorSegreto, Helena Regina Comodo [UNIFESP]
dc.contributor.authorBarrezueta, Luis Fernando Mesias [UNIFESP]
dc.contributor.authorCosta, Henrique de Oliveira [UNIFESP]
dc.contributor.authorLima, Flavio de Oliveira [UNIFESP]
dc.contributor.authorForones, Nora Manoukian [UNIFESP]
dc.contributor.authorRibeiro, Daniel Araki [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:05:59Z
dc.date.available2016-01-24T14:05:59Z
dc.date.issued2011-01-01
dc.description.abstractThe purpose of this investigation was to analyze the immunoexpression of FasL, Fas, FADD, cleaved caspase 8, and cleaved caspase 3 in gastric cancer. Formalin-fixed and paraffin-embedded gastric adenocarcinoma tissues from 87 patients, including adjacent normal tissues, were included on tissue microarray by immunohistochemistry. the tumor and the adjacent normal tissues were positive for FasL in 66.7% and 90.6%, for Fas in 52.8% and 52.4%, for FADD in 67.4% and 82.3%, for cleaved caspase 8 in 27.9% and 37.7%, and for cleaved caspase 3 in 33.7% and 8.3%, respectively. FasL and the FADD from tumor were statistically different in relation to the histological type. Cleaved caspase 8 was statistically different in relation to clinical stage (p = 0.031). the FADD from normal tissue was statistically different in relation to age (p = 0.039), sex (p = 0.055), clinical stage (p = 0.019), and Fas was different in relation to tumor size (p = 0.012). in the tumor, we observed a correlation between FasL and Fas, FasL and FADD, and FasL and cleaved caspase 3. in the adjacent normal tissue, a correlation was observed between FasL and Fas, FasL and FADD. There was no association of another marker with sex, age, clinical stage, and survival. Our results suggest that these proteins mediate the early extrinsic apoptotic pathway in gastric cancer and adjacent normal mucosa. FasL protein binds to Fas protein and subsequently binds to death receptor FADD signaling activation of the extrinsic apoptotic pathway. in this phase, there was inhibition of caspase 8 and, consequently, decreased apoptosis. (C) 2011 Elsevier GmbH. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Dept Patol, São Paulo, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Lab Radiol, Dept Med, São Paulo, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Grp Oncol,Div Gastroentererol, Dept Med, São Paulo, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biociencias, UNIFESP, São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Dept Patol, São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Lab Radiol, Dept Med, São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Grp Oncol,Div Gastroentererol, Dept Med, São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biociencias, UNIFESP, São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 04/09932-4
dc.description.sponsorshipIDFAPESP: 06/54187-0
dc.format.extent613-617
dc.identifierhttps://dx.doi.org/10.1016/j.prp.2011.06.010
dc.identifier.citationPathology Research and Practice. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 207, n. 10, p. 613-617, 2011.
dc.identifier.doi10.1016/j.prp.2011.06.010
dc.identifier.issn0344-0338
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/33288
dc.identifier.wosWOS:000297952600003
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofPathology Research and Practice
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectGastric canceren
dc.subjectTMAen
dc.subjectApoptosisen
dc.subjectImmunohistochemistryen
dc.titleThe extrinsic apoptotic signaling pathway in gastric adenocarcinomas assessed by tissue microarrayen
dc.typeinfo:eu-repo/semantics/article
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