Molecular fragmentation of wheat-germ agglutinin induced by food irradiation reduces its allergenicity in sensitised mice

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dc.contributor.authorVaz, Antonio F. M.
dc.contributor.authorSouza, Marthyna P.
dc.contributor.authorCarneiro-da-Cunha, Maria G.
dc.contributor.authorMedeiros, Paloma L.
dc.contributor.authorMelo, Ana M. M. A.
dc.contributor.authorAguiar, Jaciana S.
dc.contributor.authorSilva, Teresinha G.
dc.contributor.authorSilva-Lucca, Rosemeire Aparecida [UNIFESP]
dc.contributor.authorOliva, Maria Luiza Vilela [UNIFESP]
dc.contributor.authorCorreia, Maria T. S.
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.contributor.institutionUniv Estadual Oeste Parana
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:27:14Z
dc.date.available2016-01-24T14:27:14Z
dc.date.issued2012-05-15
dc.description.abstractWGA, an agglutinin from wheat germ which is largely responsible for many of wheat's allergies, was used as a model to investigate the action of ionising radiation on WGA's anti-nutritive effects in sensitised mice. Based on the molecular structure, the present study also examined the structural modification of WGA in relation to the range of dose. Structural integrity was monitored using HPLC, fluorescence spectrometry and circular dichroism. Results showed a loss of intrinsic activity and the formation of insoluble amorphous aggregates with a lack of native conformational structures after irradiation. Current findings suggest that the allergenic epitopes of WGA became less active and antigenic after high-dose radiation. the reduction of cytokines typical of allergic reactions, with decreased lymphocytic infiltrate, was observed in the gut of mice given irradiated versus native WGA. Food irradiation proved effective and safe in combating immunological and allergic effects of WGA. (C) 2011 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Fed Pernambuco, Dept Bioquim, Recife, PE, Brazil
dc.description.affiliationUniv Fed Pernambuco, Dept Histol & Embriol, Recife, PE, Brazil
dc.description.affiliationUniv Fed Pernambuco, Dept Biofis & Radiobiol, Recife, PE, Brazil
dc.description.affiliationUniv Fed Pernambuco, Dept Antibiot, Recife, PE, Brazil
dc.description.affiliationUniv Estadual Oeste Parana, Ctr Engn & Ciencias Exatas, Toledo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Bioquim, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Bioquim, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipMinisterio da Ciencia e Tecnologia (Brazilian)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE)
dc.format.extent1033-1039
dc.identifierhttp://dx.doi.org/10.1016/j.foodchem.2011.11.097
dc.identifier.citationFood Chemistry. Oxford: Elsevier B.V., v. 132, n. 2, p. 1033-1039, 2012.
dc.identifier.doi10.1016/j.foodchem.2011.11.097
dc.identifier.fileWOS000300475800050.pdf
dc.identifier.issn0308-8146
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/34893
dc.identifier.wosWOS:000300475800050
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofFood Chemistry
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectFood allergensen
dc.subjectAnti-nutritive effecten
dc.subjectFood irradiationen
dc.subjectWGAen
dc.titleMolecular fragmentation of wheat-germ agglutinin induced by food irradiation reduces its allergenicity in sensitised miceen
dc.typeinfo:eu-repo/semantics/article
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