Amifostine Increases FAS and Caspase-3 Expression in Colonic Tissue of Irradiated Mice

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dc.contributor.authorOshima, Celina Tizuko Fujiyama [UNIFESP]
dc.contributor.authorRibeiro, Daniel Araki [UNIFESP]
dc.contributor.authorGomes, Thiago Simao [UNIFESP]
dc.contributor.authorAdios, Priscila C. [UNIFESP]
dc.contributor.authorEgami, Mizue Imoto [UNIFESP]
dc.contributor.authorSegreto, Helena Regina Comodo [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2018-06-15T13:50:16Z
dc.date.available2018-06-15T13:50:16Z
dc.date.issued2015-05-01
dc.description.abstractBackground/Aim: The aim of this study was to evaluate the expression of FASL, FAS and FADD and caspase-3 in oesophagus, stomach and colonic tissues of mice irradiated in vivo by immunohistochemistry. Materials and Methods: A total of 48 adult male C57BL mice were distributed into four groups: Ami(-)/Rad(-): Mice received 0.5 ml of 0.9% physiological saline solution (PPS) intraperitioneally (i.p.); Ami(+)/Rad(-): mice received amifostine (400mg/kg i.p.) freshly dissolved in double-distilled water; Ami(-)/Rad(+): mice received 0.5 ml of PSS i.p. 30 min before a single whole-body radiation dose of 7 Gy; Ami(+)/Rad(+): mice received 0.5 ml of an aqueous solution of 400 mg/kg amifostine i.p.30 min prior to irradiation. All groups were assigned into subgroups sacrificed at 0.5 h, 1 h, 2 h and 4 h after irradiation. Results. In oesophagus and stomach tissues, we did not observe any difference between Ami(-)/ad(-), Ami(+)/Rad(-), Ami(-)/Rad(+) and Ami(+)/Rad(+) groups in the expression of FASL, FAS and FADD. The colonic tissue was the only to exhibit any difference in the expression of FAS and caspase-3 protein in the Ami(-)/Rad(+) group at 1 and 2 h. Amifostine increased FAS and caspase-3 immunoexpression when compared to the control. Immunoexpression for FASL and FADD was not remarkably different in colonic tissue. Conclusion: Taken together, our results demonstrate that amifostine increases FAS and caspase-3 expression in colonic tissue of irradiated mice.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Pathol, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biosci, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Clin & Expt Oncol, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Morphol, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Pathol, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biosci, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Clin & Expt Oncol, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Morphol, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent2817-2822
dc.identifierhttps://ar.iiarjournals.org/content/35/5/2817.abstract
dc.identifier.citationAnticancer Research. Athens: Int Inst Anticancer Research, v. 35, n. 5, p. 2817-2822, 2015.
dc.identifier.issn0250-7005
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/42604
dc.identifier.wosWOS:000354267200041
dc.language.isoeng
dc.publisherInt Inst Anticancer Research
dc.relation.ispartofAnticancer Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectAmifostineen
dc.subjectRadiationen
dc.subjectMouseen
dc.subjectApoptosisen
dc.titleAmifostine Increases FAS and Caspase-3 Expression in Colonic Tissue of Irradiated Miceen
dc.typeinfo:eu-repo/semantics/article
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