The pan HLA DR-binding epitope improves adjuvant-assisted immunization with a recombinant protein containing a malaria vaccine candidate

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dc.contributor.authorRosa, D. S.
dc.contributor.authorTzelepis, F.
dc.contributor.authorCunha, M. G.
dc.contributor.authorSoares, I. S.
dc.contributor.authorRodrigues, M. M.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFed Univ Para
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T12:37:06Z
dc.date.available2016-01-24T12:37:06Z
dc.date.issued2004-04-15
dc.description.abstractThe pan HLA DR-binding epitope (PADRE) has been proposed as a simple carrier epitope suitable for use in the development of synthetic and recombinant vaccines. Using the mouse model, we evaluated whether PADRE could improve adjuvant-assisted immunizations with a recombinant malarial protein containing the 19 kDa C-terminal region of merozoite surface protein 1 (MSP1(19)) that is a Plasmodium vivax vaccine candidate. Initially, the antibody immune response was evaluated in C57BL/6 mice, a mouse strain which develops a strong T cell immune response to PADRE. When administered in distinct adjuvant formulations, antibody titers induced by the recombinant protein His(6)MSP1(19)-PADRE were not significantly different to those generated by complete/incomplete Freund's adjuvant (CFA/IFA) in terms of magnitude, affinity, IgG subclasses and longevity. However, in C57BL/6 mice immunized with the recombinant protein His(6)MSP1(19), strong antibody responses could be generated in the presence of CFA/IFA but not other classes of adjuvants such as CpG ODN 1826 or MPL/TDM. Similarly, in BALB/c mice that do not develop T cells specific for PADRE, the recombinant protein His(6)MSP1(19)-PADRE failed to induce high antibody titers in the presence of adjuvants other than CFA/IFA. Our results indicated that when adjuvants that are not as strong as CFA/IFA are employed, the presence of PADRE greatly improved adjuvant-assisted antibody immune responses induced by a malarial recombinant antigen. Considering the great limitations of adjuvants for human use, our observation may improve the rational design of new vaccine formulations. (C) 2004 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationFed Univ Para, Dept Patol, Ctr Ciencias Biol, BR-66075900 Belem, Para, Brazil
dc.description.affiliationUniv São Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, BR-05508900 São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent259-268
dc.identifierhttp://dx.doi.org/10.1016/j.imlet.2004.01.006
dc.identifier.citationImmunology Letters. Amsterdam: Elsevier B.V., v. 92, n. 3, p. 259-268, 2004.
dc.identifier.doi10.1016/j.imlet.2004.01.006
dc.identifier.issn0165-2478
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/27709
dc.identifier.wosWOS:000221166600010
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofImmunology Letters
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectpan HLA DR-binding epitopeen
dc.subjectrecombinant vaccines and adjuvantsen
dc.titleThe pan HLA DR-binding epitope improves adjuvant-assisted immunization with a recombinant protein containing a malaria vaccine candidateen
dc.typeinfo:eu-repo/semantics/article
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