CHEMILUMINESCENT IMMUNOASSAYS - DISCRIMINATION BETWEEN the REACTIVITIES of NATURAL and HUMAN PATIENT ANTIBODIES WITH ANTIGENS FROM EUKARYOTIC PATHOGENS, TRYPANOSOMA-CRUZI and PARACOCCIDIOIDES-BRASILIENSIS

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dc.contributor.authorAlmeida, I. C.
dc.contributor.authorRodrigues, E. G.
dc.contributor.authorTravassos, L. R.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T11:40:15Z
dc.date.available2016-01-24T11:40:15Z
dc.date.issued1994-01-01
dc.description.abstractQuantitative chemiluminescent enzyme-linked immunosorbent assay (ELISA) and dot-blotting procedures were developed to evaluate the reactivity of human antibodies with crude antigens and purified molecules of parasites and fungi, mainly Trypanosoma cruzi and Paracoccidioides brasiliensis. Reproducible, highly sensitive, and strictly dose-responding results were obtained, with the specificity depending on the kind of antigen used. Mixed antigens (epimastigote membrane and HIV-I heptapeptide) applied in dots could be independently recognized by specific sera. Purified antigens (T. cruzi F2/3 and P. brasiliensis gp43) at very small concentrations gave specific reactions with patients' sera diluted greater than or equal to 1:1,000 and were very poorly reactive or unreactive with natural antibodies using the chemiluminescent immunoassays. P. brasiliensis crude antigen Fava Netto polysaccharide antigen (FNPA) contained peptide epitopes recognized by natural antibodies and carbohydrate epitopes reactive with sera from histoplasmosis patients. It is very important that sensitive chemiluminescence immunoassays be used with purified antigenic molecules to ensure specificity for the diagnosis and follow-up of parasitic and fungal infections. (C) 1994 Wiley-Liss, Inc.en
dc.description.affiliationESCOLA PAULISTA MED,DISCIPLINA BIOL CELULAR,BR-04023 São Paulo,BRAZIL
dc.description.affiliationUnifespESCOLA PAULISTA MED,DISCIPLINA BIOL CELULAR,BR-04023 São Paulo,BRAZIL
dc.description.sourceWeb of Science
dc.format.extent424-431
dc.identifierhttp://dx.doi.org/10.1002/jcla.1860080614
dc.identifier.citationJournal of Clinical Laboratory Analysis. New York: Wiley-liss, v. 8, n. 6, p. 424-431, 1994.
dc.identifier.doi10.1002/jcla.1860080614
dc.identifier.issn0887-8013
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/25372
dc.identifier.wosWOS:A1994QF65200013
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Clinical Laboratory Analysis
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectCHEMILUMINESCENT ELISAen
dc.subjectCHEMILUMINESCENT DOT-BLOTTINGen
dc.subjectAMERICAN TRYPANOSOMIASISen
dc.subjectPARACOCCIDIOIDOMYCOSISen
dc.subjectMUCIN-LIKE GLYCOCONJUGATESen
dc.subjectEPIMASTIGOTE MEMBRANEen
dc.subjectPOLYSACCHARIDE ANTIGENen
dc.subject43-KDA GLYCOPROTEINen
dc.titleCHEMILUMINESCENT IMMUNOASSAYS - DISCRIMINATION BETWEEN the REACTIVITIES of NATURAL and HUMAN PATIENT ANTIBODIES WITH ANTIGENS FROM EUKARYOTIC PATHOGENS, TRYPANOSOMA-CRUZI and PARACOCCIDIOIDES-BRASILIENSISen
dc.typeinfo:eu-repo/semantics/article
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