The Value of Specific IgE to Peanut and Its Component Ara h 2 in the Diagnosis of Peanut Allergy

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dc.contributor.authorLopes de Oliveira, Lucila C. [UNIFESP]
dc.contributor.authorAderhold, Martina
dc.contributor.authorBrill, Marianne
dc.contributor.authorSchulz, Gabriele
dc.contributor.authorRolinck-Werninghaus, Claudia
dc.contributor.authorMills, E. N. Clare
dc.contributor.authorNiggemann, Bodo
dc.contributor.authorNaspitz, Charles Kirov [UNIFESP]
dc.contributor.authorWahn, Ulrich
dc.contributor.authorBeyer, Kirsten
dc.contributor.institutionCharite
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionVBC Genom
dc.contributor.institutionInst Food Res
dc.contributor.institutionGerman Red Cross Hosp Westend
dc.date.accessioned2016-01-24T14:31:57Z
dc.date.available2016-01-24T14:31:57Z
dc.date.issued2013-07-01
dc.description.abstractBACKGROUND: To avoid unnecessary oral food challenges, which are time consuming, stressful, and risky, improved in vitro diagnostic methods for food allergy such as component resolved diagnostics are still under investigation.OBJECTIVE: To investigate the role of whole peanut-and peanut-component (Ara h 1, Ara h 2, Ara h 3, Ara h 6 and Ara h 8)-specific IgE levels in the diagnostic procedure of peanut allergy as well as the diagnostic properties of peanut-specific IgG and IgG(4).METHODS: Sixty-one children underwent oral peanut challenge tests for diagnostic purposes irrespective of their peanut-specific IgE levels. Peanut-specific serum IgE, IgG, and IgG(4) levels were determined by ImmunoCAP FEIA and specific IgE against individual peanut proteins by Immuno Solid-phase Allergen Chip.RESULTS: Thirty-four of 61 patients (56%) had a peanut allergy. No significant difference was observed for peanut-specific IgG or peanut-specific IgG(4) levels between patients who were allergic and tolerant patients, whereas peanut-specific IgE was significant higher in patients who were allergic than in tolerant patients (P < .005). Twenty-five of 61 children had peanut-specific IgE above a previously proposed cutoff level of 15 kU(A)/L; however, 7 of these 25 children (28%) were clinically tolerant. Ara h 2-specific IgE was significantly lower in tolerant than in patients with allergies (P < .0001). Interestingly, 94% of the patients with peanut allergies showed IgE-binding to Ara h 2. Unfortunately, 26% of the sensitized but tolerant patients have shown IgE-binding to Ara h 2 too.CONCLUSIONS: Neither the level of specific IgE to peanut nor to Ara h 2 was able to clearly distinguish patients with clinical relevant peanut allergy from those who were clinical tolerant in our population. As expected, peanut-specific IgG and IgG(4) did not improve the diagnostic procedure. (C) 2013 American Academy of Allergy, Asthma & Immunologyen
dc.description.affiliationCharite, Dept Pediat Pneumol & Immunol, D-13353 Berlin, Germany
dc.description.affiliationUniversidade Federal de São Paulo, Dept Pediat, Div Allergy & Clin Immunol, São Paulo, Brazil
dc.description.affiliationVBC Genom, Vienna, Austria
dc.description.affiliationInst Food Res, Norwich NR4 7UA, Norfolk, England
dc.description.affiliationGerman Red Cross Hosp Westend, Pediat Allergol & Pneumol, Berlin, Germany
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Pediat, Div Allergy & Clin Immunol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipEuropean Commission
dc.description.sponsorshipGerman Research Foundation
dc.description.sponsorshipIDEuropean Commission: FOOD-CT-2005-514000
dc.description.sponsorshipIDGerman Research Foundation: DFG-BE3991/1-1
dc.format.extent394-398
dc.identifierhttp://dx.doi.org/10.1016/j.jaip.2013.05.010
dc.identifier.citationJournal of Allergy and Clinical Immunology-in Practice. Amsterdam: Elsevier B.V., v. 1, n. 4, p. 394-398, 2013.
dc.identifier.doi10.1016/j.jaip.2013.05.010
dc.identifier.issn2213-2198
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36489
dc.identifier.wosWOS:000209374500011
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Allergy and Clinical Immunology-in Practice
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectIgEen
dc.subjectIgGen
dc.subjectIgG(4)en
dc.subjectPeanut allergyen
dc.subjectImmuno Solid-phase Allergen Chipen
dc.subjectComponent-resolved diagnosticsen
dc.subjectAra h 1en
dc.subjectAra h 2en
dc.subjectAra h 3en
dc.subjectAra h 6en
dc.subjectAra h 8en
dc.titleThe Value of Specific IgE to Peanut and Its Component Ara h 2 in the Diagnosis of Peanut Allergyen
dc.typeinfo:eu-repo/semantics/article
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