Cloning, Characterization and Anti-Inflammatory Properties of Bothrops jararaca Snake Antithrombin

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dc.contributor.authorMorais-Zani, Karen de
dc.contributor.authorGrego, Kathleen Fernandes
dc.contributor.authorTorquato, Ricardo José Soares [UNIFESP]
dc.contributor.authorSilva, Caroline Serino
dc.contributor.authorTanaka, Aparecida Sadae [UNIFESP]
dc.contributor.authorTanaka-Azevedo, Anita Mitico
dc.contributor.institutionInst Butantan
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2018-06-18T11:27:17Z
dc.date.available2018-06-18T11:27:17Z
dc.date.issued2015-01-01
dc.description.abstractAntithrombin inhibits blood coagulation through the interaction with serine proteases in both intrinsic and extrinsic pathways. In addition, antithrombin also shows anti-inflammatory properties, which are independent of its effects on coagulation. This work shows for the first time the cloning and sequencing of antithrombin from a snake species. This predicted protein is composed by 430 amino acids and presents about 64.5% sequence identity to human antithrombin. Biacore experiments revealed that the binding affinity of Bothrops jararaca snake antithrombin to heparin was similar to 30 times higher than that of human antithrombin. Furthermore, Bothrops jararaca antithrombin is more effective in preventing acute inflammation induced by carrageenan when compared to human antithrombin. Hence, the results showed herein suggest that Bothrops jararaca antithrombin can play a key role in the control of acute inflammation and that this molecule might be used as a pharmacological tool and as a prototype for drug development.en
dc.description.affiliationInst Butantan, Lab Herpetol, BR-05503900 Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Inst Butantan, Inst Pesquisas Tecnol, Programa Posgrad Interunidades Biotecnol, BR-05508900 Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Bioquim, BR-04044020 Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Bioquim, BR-04044020 Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 2009/03484-3
dc.description.sponsorshipIDFAPESP: 2009/50199-2
dc.description.sponsorshipIDFAPESP: 2009/53844-6
dc.description.sponsorshipIDFAPESP: 2011/21558-4
dc.format.extent410-418
dc.identifierhttp://dx.doi.org/10.2174/0929866522666150216150704
dc.identifier.citationProtein And Peptide Letters. Sharjah: Bentham Science Publ Ltd, v. 22, n. 5, p. 410-418, 2015.
dc.identifier.doi10.2174/0929866522666150216150704
dc.identifier.issn0929-8665
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/45153
dc.identifier.wosWOS:000354338300004
dc.language.isoeng
dc.publisherBentham Science Publ Ltd
dc.relation.ispartofProtein And Peptide Letters
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectAntithrombinen
dc.subjectBothrops jararaca snakeen
dc.subjectcarrageenanen
dc.subjectcloningen
dc.subjectheparin affinityen
dc.subjectinflammationen
dc.subjectsurface plasmon resonanceen
dc.titleCloning, Characterization and Anti-Inflammatory Properties of Bothrops jararaca Snake Antithrombinen
dc.typeinfo:eu-repo/semantics/article
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