Elastase-2, a Tissue Alternative Pathway for Angiotensin II Generation, Plays a Role in Circulatory Sympathovagal Balance in Mice

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dc.citation.volume8
dc.contributor.authorBecari, Christiane
dc.contributor.authorDurand, Marina T.
dc.contributor.authorGuimaraes, Alessander O. [UNIFESP]
dc.contributor.authorLataro, Renata M.
dc.contributor.authorPrado, Cibele M.
dc.contributor.authorde Oliveira, Mauro
dc.contributor.authorCandido, Sarai C. O.
dc.contributor.authorPais, Paloma
dc.contributor.authorRibeiro, Mauricio S.
dc.contributor.authorBader, Michael
dc.contributor.authorPesquero, Joao B. [UNIFESP]
dc.contributor.authorSalgado, Maria C. O.
dc.contributor.authorSalgado, Helio C.
dc.coverageLausanne
dc.date.accessioned2020-07-17T14:02:38Z
dc.date.available2020-07-17T14:02:38Z
dc.date.issued2017
dc.description.abstractIn vitro and ex vivo experiments indicate that elastase-2 (ELA-2), a chymotrypsin-serine protease elastase family member 2A, is an alternative pathway for angiotensin II (Ang II) generation. However, the role played by ELA-2 in vivo is unclear. We examined ELA-2 knockout (ELA-2K0) mice compared to wild-type (WT) mice and determined whether ELA-2 played a role in hemodynamics [arterial pressure (AP) and heart rate (HR)], cardiocirculatory sympathovagal balance and baroreflex sensitivity. The variability of systolic arterial pressure (SAP) and pulse interval (PI) for evaluating autonomic modulation was examined for time and frequency domains (spectral analysis), whereas a symbolic analysis was also used to evaluate PI variability. In addition, baroreflex sensitivity was examined using the sequence method. Cardiac function was evaluated echocardiographically under anesthesia. The AP was normal whereas the HR was reduced in ELA-2K0 mice (425 +/- 17 vs. 512 +/- 13 bpm from WT). SAP variability and baroreflex sensitivity were similar in both strains. The LF power from the PI spectrum (33.6 +/- 5 vs. 51.8 +/- 4.8 nu from WT) and the LF/HF ratio (0.60 +/- 0.1 vs. 1.45 +/- 0.3 from WT) were reduced, whereas the HF power was increased (66.4 +/- 5 vs. 48.2 +/- 4.8 nu from WI) in ELA-2K0 mice, indicating a shift toward parasympathetic modulation of HR. Echocardiographic examination showed normal fractional shortening and an ejection fraction in ELA-2K0 miceen
dc.description.abstracthowever, the cardiac output, stroke volume, and ventricular size were reduced. These findings provide the first evidence that ELA-2 acts on the sympathovagal balance of the heart, as expressed by the reduced sympathetic modulation of HR in ELA-2K0 mice.en
dc.description.affiliationUniv Sao Paulo, Ribeirao Preto Med Sch, Dept Physiol, Ribeirao Preto, Brazil
dc.description.affiliationUniv Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, Ribeirao Preto, Brazil
dc.description.affiliationMayo Clin, Dept Cardiovasc Dis, Rochester, MN USA
dc.description.affiliationUniv Ribeirao Preto, Dept Med, Ribeirao Preto, Brazil
dc.description.affiliationMax Delbruck Ctr Mol Med, Berlin, Germany
dc.description.affiliationUniv Fed Sao Paulo, Dept Biophys, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Ribeirao Preto Med Sch, Dept Pathol, Ribeirao Preto, Brazil
dc.description.affiliationUniv Sao Paulo, Ribeirao Preto Med Sch, Dept Surg & Anat, Ribeirao Preto, Brazil
dc.description.affiliationCharite, Berlin Inst Hlth, Berlin, Germany
dc.description.affiliationGerman Canc Cardiovasc Res, Parter Site Berlin, Berlin, Germany
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biophys, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientffico e Tecnologico (CNPq), Brazil
dc.description.sponsorshipFAPESP
dc.description.sponsorshipIDFAPESP: 2013/20549-7
dc.description.sponsorshipIDFAPESP: 2009/50548-7
dc.description.sponsorshipIDCNPq: 475715/2012-8
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.3389/fphys.2017.00170
dc.identifier.citationFrontiers In Physiology. Lausanne, v. 8, p. -, 2017.
dc.identifier.doi10.3389/fphys.2017.00170
dc.identifier.fileWOS000397087400001.pdf
dc.identifier.issn1664-042X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54913
dc.identifier.wosWOS:000397087400001
dc.language.isoeng
dc.publisherFrontiers Media Sa
dc.relation.ispartofFrontiers In Physiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectelastase-2en
dc.subjectangiotensina IIen
dc.subjectsympathovagal balanceen
dc.subjectanalysis spectralen
dc.subjectknockout miceen
dc.titleElastase-2, a Tissue Alternative Pathway for Angiotensin II Generation, Plays a Role in Circulatory Sympathovagal Balance in Miceen
dc.typeinfo:eu-repo/semantics/article
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