Primary Human Uterine Leiomyoma Cell Culture Quality Control: Some Properties of Myometrial Cells Cultured under Serum Deprivation Conditions in the Presence of Ovarian Steroids

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dc.citation.issue7
dc.citation.volume11
dc.contributor.authorBonazza, Camila [UNIFESP]
dc.contributor.authorAndrade, Sheila Siqueira [UNIFESP]
dc.contributor.authorSumikawa, Joana Tomomi [UNIFESP]
dc.contributor.authorBatista, Fabricio Pereira [UNIFESP]
dc.contributor.authorParedes-Gamero, Edgar J. [UNIFESP]
dc.contributor.authorGirao, Manoel J. B. C. [UNIFESP]
dc.contributor.authorOliva, Maria Luiza V. [UNIFESP]
dc.contributor.authorCastro, Rodrigo Aquino [UNIFESP]
dc.coverageSan Francisco
dc.date.accessioned2020-08-14T13:44:14Z
dc.date.available2020-08-14T13:44:14Z
dc.date.issued2016
dc.description.abstractCell culture is considered the standard media used in research to emulate the in vivo cell environment. Crucial in vivo experiments cannot be conducted in humans and depend on in vitro methodologies such as cell culture systems. However, some procedures involving the quality control of cells in culture have been gradually neglected by failing to acknowledge that primary cells and cell lines change over time in culture. Thus, we report methods based on our experience for monitoring primary cell culture of human myometrial cells derived from uterine leiomyoma. We standardized the best procedure of tissue dissociation required for the study of multiple genetic marker systems that include species-specific antigens, expression of myofibroblast or myoblast markers, growth curve, serum deprivation, starvation by cell cycle synchronization, culture on collagen coated plates, and 17 beta-estradiol (E-2) and progesterone (P-4) effects. The results showed that primary myometrial cells from patients with uterine leiomyoma displayed myoblast phenotypes before and after in vitro cultivation, and leiomyoma cells differentiated into mature myocyte cells under the appropriate differentiation-inducing conditions (serum deprivation). These cells grew well on collagen coated plates and responded to E2 and P4, which may drive myometrial and leiomyoma cells to proliferate and adhere into a focal adhesion complex involvement in a paracrine manner. The establishment of these techniques as routine procedures will improve the understanding of the myometrial physiology and pathogenesis of myometrium-derived diseases such as leiomyoma. Mimicking the in vivo environment of fibrotic conditions can prevent false results and enhance results that are based on cell culture integrity.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Gynecol, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biochem, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Gynecol, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biochem, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipAssociacao Beneficente de Coleta de Sangue (Colsan)
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
dc.description.sponsorshipIDFAPESP: 2012/19780-3
dc.description.sponsorshipIDFAPESP: 2012/19851-8
dc.description.sponsorshipIDFAPESP: 2009/53766-5
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0158578
dc.identifier.citationPlos One. San Francisco, v. 11, n. 7, p. -, 2016.
dc.identifier.doi10.1371/journal.pone.0158578
dc.identifier.fileWOS000380005400082.pdf
dc.identifier.issn1932-6203
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57548
dc.identifier.wosWOS:000380005400082
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titlePrimary Human Uterine Leiomyoma Cell Culture Quality Control: Some Properties of Myometrial Cells Cultured under Serum Deprivation Conditions in the Presence of Ovarian Steroidsen
dc.typeinfo:eu-repo/semantics/article
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