Identification of IL11RA and MELK amplification in gastric cancer by comprehensive genomic profiling of gastric cancer cell lines

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dc.citation.issue43
dc.citation.volume22
dc.contributor.authorCalcagno, Danielle Queiroz [UNIFESP]
dc.contributor.authorTakeno, Sylvia Santomi [UNIFESP]
dc.contributor.authorGigek, Carolina Oliveira [UNIFESP]
dc.contributor.authorLeal, Mariana Ferreira [UNIFESP]
dc.contributor.authorWisnieski, Fernanda [UNIFESP]
dc.contributor.authorChen, Elizabeth Suchi [UNIFESP]
dc.contributor.authorAraújo, Taíssa Maíra Thomaz [UNIFESP]
dc.contributor.authorLima, Eleonidas Moura [UNIFESP]
dc.contributor.authorMelaragno, Maria Isabel [UNIFESP]
dc.contributor.authorDemachki, Samia [UNIFESP]
dc.contributor.authorAssumpção, Paulo Pimentel [UNIFESP]
dc.contributor.authorBurbano, Rommel Rodríguez [UNIFESP]
dc.contributor.authorSmith, Marilia de Arruda Cardoso [UNIFESP]
dc.coveragePleasanton
dc.date.accessioned2020-07-31T12:47:16Z
dc.date.available2020-07-31T12:47:16Z
dc.date.issued2016
dc.description.abstractAIM To identify common copy number alterations on gastric cancer cell lines. METHODS Four gastric cancer cell lines (ACP02, ACP03, AGP01 and PG100) underwent chromosomal comparative genome hybridization and array comparative genome hybridization. We also confirmed the results by fluorescence in situ hybridization analysis using the bacterial artificial chromosome clone and quantitative real time PCR analysis. RESULTS The amplification of 9p13.3 was detected in all cell lines by both methodologies. An increase in the copy number of 9p13.3 was also confirmed by fluorescence in situ hybridization analysis. Moreover, the interleukin 11 receptor alpha (IL11RA) and maternal embryonic leucine zipper kinase (MELK) genes, which are present in the 9p13.3 amplicon, revealed gains of the MELK gene in all the cell lines studied. Additionally, a gain in the copy number of IL11RA and MELK was observed in 19.1% (13/68) and 55.9% (38/68) of primary gastric adenocarcinoma samples, respectively. CONCLUSION The characterization of a small gain region at 9p13.3 in gastric cancer cell lines and primary gastric adenocarcinoma samples has revealed MELK as a candidate target gene that is possibly related to the development of gastric cancer.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Morfol & Genet, Disciplina Genet, BR-04021001 Sao Paulo, SP, Brazil
dc.description.affiliationHosp Univ Joao Barros Barreto, Nucleo Pesquisas Oncol, Av Mundurucus 4487,1 Piso Unacon, BR-66073000 Belem, PA, Brazil
dc.description.affiliationUniv Fed Paraiba, Dept Biol Mol, BR-58051900 Joao Pessoa, Paraiba, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Disciplina Gastroenterol Cirurg, BR-04021001 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Ortopedia & Traumatol, BR-04021001 Sao Paulo, SP, Brazil
dc.description.affiliationUnifespDisciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, São Paulo, SP 04021-001, Brazil
dc.description.affiliationUnifespDisciplina de Gastroenterologia Cirúrgica, Universidade Federal de São Paulo, São Paulo, SP 04021-001, Brazil
dc.description.affiliationUnifespDepartamento de Ortopedia e Traumatologia, Universidade Federal de São Paulo, São Paulo, SP 04021-001, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2009/07145-9
dc.format.extent9506-9514
dc.identifierhttp://dx.doi.org/10.3748/wjg.v22.i43.9506
dc.identifier.citationWorld Journal Of Gastroenterology. Pleasanton, v. 22, n. 43, p. 9506-9514, 2016.
dc.identifier.doi10.3748/wjg.v22.i43.9506
dc.identifier.fileWOS000389203400005.pdf
dc.identifier.issn1007-9327
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56706
dc.identifier.wosWOS:000389203400005
dc.language.isoeng
dc.publisherBaishideng Publishing Group Inc
dc.relation.ispartofWorld Journal Of Gastroenterology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectIL11RAen
dc.subjectGastric canceren
dc.subjectGenomic profilingen
dc.subjectMELKen
dc.subject9p13.3en
dc.titleIdentification of IL11RA and MELK amplification in gastric cancer by comprehensive genomic profiling of gastric cancer cell linesen
dc.typeinfo:eu-repo/semantics/article
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