Multicenter evaluation of mycobacteria identification by PCR restriction enzyme analysis in laboratories from Latin America and the Caribbean

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dc.contributor.authorLeao, S. C.
dc.contributor.authorBernardelli, A.
dc.contributor.authorCataldi, A.
dc.contributor.authorZumarraga, M.
dc.contributor.authorRobledo, J.
dc.contributor.authorRealpe, T.
dc.contributor.authorMejia, G. I.
dc.contributor.authorTelles, MAD
dc.contributor.authorChimara, E.
dc.contributor.authorVelazco, M.
dc.contributor.authorFernandez, J.
dc.contributor.authorRodrigues, P. A.
dc.contributor.authorGuerrero, M. I.
dc.contributor.authorLeon, C. I.
dc.contributor.authorPorras, T. B.
dc.contributor.authorRastogi, N.
dc.contributor.authorGoh, K. S.
dc.contributor.authorSuffys, P.
dc.contributor.authorRocha, A. D.
dc.contributor.authorNetto, D. D.
dc.contributor.authorRitacco, V
dc.contributor.authorLopez, B.
dc.contributor.authorBarrera, L.
dc.contributor.authorPalomino, J. C.
dc.contributor.authorMartin, A.
dc.contributor.authorPortaels, F.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionSENASA
dc.contributor.institutionINTA
dc.contributor.institutionCorp Invest Biol
dc.contributor.institutionUniv Pontificia Bolivariana
dc.contributor.institutionInst Adolfo Lutz Registro
dc.contributor.institutionInst Salud Publ Chile
dc.contributor.institutionInst Nacl Salud
dc.contributor.institutionInst Pasteur Guadeloupe
dc.contributor.institutionFdn Oswaldo Cruz
dc.contributor.institutionInst Nacl Enfermedades Infecciosas Carlos Malbran
dc.contributor.institutionInst Trop Med
dc.date.accessioned2016-01-24T12:37:50Z
dc.date.available2016-01-24T12:37:50Z
dc.date.issued2005-05-01
dc.description.abstractThe identification of mycobacterial species in clinical isolates is essential for making patient care decisions. Polymerase chain reaction (PCR) restriction enzyme analysis (PRA) is a simple and rapid identification method, based on amplification of 441 bp of the hsp65 gene and restriction with BstEII and HaeIII. As a contribution to the validation of PRA, a multicenter study was performed in eight laboratories located in Argentina, Brazil, Colombia, Chile, and Guadeloupe. Each laboratory received 18 coded isolates from the collection of the Institute of Tropical Medicine (Antwerp, Belgium), representing duplicates of nine laboratory strains: Mycobacterium terrae CIPT 140320001, Mycobacterium scrofulaceum CIPT 140220031, Mycobacterium flavescens ATCC 14474, Mycobacterium triviale ATCC 23292, Mycobacterium nonchromogenicum ATCC 19530, Mycobacterium chitae ATCC 19627, Mycobacterium abscessus ATCC 19977, Mycobacterium kansasii ATCC 12478, and Mycobacterium peregrinum ATCC 14467. A detailed protocol including amplification, enzymatic digestion, and gel preparation was provided to each laboratory. Two laboratories identified correctly all 18 (100%) isolates, one identified correctly 17 (94.5%), two identified 14 (77.7%), one identified 11 (61%), and two identified 8 (44.4%) isolates. Errors detected in laboratories with more than 77% accuracy were associated with electrophoresis running conditions and an unspecific amplicon produced by a single strain. Lower accuracy was mainly related to inappropriate use of DNA markers and insufficient training in interpretation of patterns. in conclusion, the PRA method was readily implemented in some Latin American and Caribbean laboratories of mycobacteria, but improvements in critical points, as gel running conditions and training in interpretation of patterns, are needed in order to improve accuracy. in others, improvement in critical points is still necessary. (C) 2004 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationSENASA, RA-1640 San Isidro, Argentina
dc.description.affiliationINTA, Inst Biotecnol, Castelar, Argentina
dc.description.affiliationCorp Invest Biol, Medellin, Colombia
dc.description.affiliationUniv Pontificia Bolivariana, Escuela Ciencias Salud, Medellin, Colombia
dc.description.affiliationInst Adolfo Lutz Registro, BR-01246902 São Paulo, Brazil
dc.description.affiliationInst Salud Publ Chile, Santiago, Chile
dc.description.affiliationInst Nacl Salud, Bogota, DC, Colombia
dc.description.affiliationInst Pasteur Guadeloupe, Unite TB & Mycobacteries, F-97165 Guadeloupe, France
dc.description.affiliationFdn Oswaldo Cruz, Inst Oswaldo Cruz, BR-21045900 Rio de Janeiro, Brazil
dc.description.affiliationInst Nacl Enfermedades Infecciosas Carlos Malbran, Buenos Aires, DF, Argentina
dc.description.affiliationInst Trop Med, B-2000 Antwerp, Belgium
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent193-199
dc.identifierhttp://dx.doi.org/10.1016/j.mimet.2004.11.015
dc.identifier.citationJournal of Microbiological Methods. Amsterdam: Elsevier B.V., v. 61, n. 2, p. 193-199, 2005.
dc.identifier.doi10.1016/j.mimet.2004.11.015
dc.identifier.issn0167-7012
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28279
dc.identifier.wosWOS:000227509000006
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Microbiological Methods
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectMycobacteriumen
dc.subjectspecies identificationen
dc.subjectPCRen
dc.titleMulticenter evaluation of mycobacteria identification by PCR restriction enzyme analysis in laboratories from Latin America and the Caribbeanen
dc.typeinfo:eu-repo/semantics/article
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