Biomonitoring of oral epithelial cells in petrol station attendants: Comparison between buccal mucosa and lateral border of the tongue

Página do item simplificado
dc.contributor.authorMartins, Renato de Almeida [UNIFESP]
dc.contributor.authorGomes, Guilherme Augusto da Silva [UNIFESP]
dc.contributor.authorAguiar, Odair [UNIFESP]
dc.contributor.authorRibeiro, Daniel Araki [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:58:47Z
dc.date.available2016-01-24T13:58:47Z
dc.date.issued2009-10-01
dc.description.abstractOwing to the influence of geno- and cytotoxicity on chemical carcinogenesis, studies have demonstrated that petroleum derivatives are able to induce genetic damage and cellular death with conflicting results so far. the aim of the present study was to comparatively evaluate DNA damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) in exfoliated oral mucosa cells from gas petrol attendants using two different anatomic buccal sites: cheek mucosa and lateral border of the tongue. A total of 23 gas petrol attendants and 23 health controls (non-exposed individuals) were included in this setting. Individuals had epithelial cells from cheek and lateral border of the tongue mechanically exfoliated, placed in fixative and dropped in clean slides which were checked for the above nuclear phenotypes. the results pointed out significant statistical differences (p<0.05) of micronucleated oral mucosa cells from gas petrol attendants for both oral sites evaluated. in the same way, petroleum derivate exposure was able to increase other nuclear alterations closely related to cytotoxicity such as karyorrhexis, pyknosis and karyolysis, being the most pronunciated effects as those found in the lateral border of the tongue. No interaction was observed between smoking and petroleum exposure. in summary, these data indicate that gas petrol attendants comprise a high risk group for DNA damage and cellular death. It seems that the lateral border of the tongue is a more sensitive site to geno- and cytotoxic insult induced by petroleum derivates. (C) 2009 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biociencias, UNIFESP, BR-11060001 Santos, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biociencias, UNIFESP, BR-11060001 Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 2007/00345-7
dc.description.sponsorshipIDFAPESP: 2007/01228-4
dc.format.extent1062-1065
dc.identifierhttps://dx.doi.org/10.1016/j.envint.2009.06.001
dc.identifier.citationEnvironment International. Oxford: Pergamon-Elsevier B.V., v. 35, n. 7, p. 1062-1065, 2009.
dc.identifier.doi10.1016/j.envint.2009.06.001
dc.identifier.issn0160-4120
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/31857
dc.identifier.wosWOS:000270312600010
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofEnvironment International
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectBuccal mucosa cellsen
dc.subjectTongue cellsen
dc.subjectPetroleum derivatesen
dc.subjectMiconucleus testen
dc.titleBiomonitoring of oral epithelial cells in petrol station attendants: Comparison between buccal mucosa and lateral border of the tongueen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
ISS - Artigos