Systemic expression of inflammatory mediators in patients with chronic rhinosinusitis and nasal polyps with and without Aspirin Exacerbated Respiratory Disease

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dc.contributor.authorPezato, Rogerio [UNIFESP]
dc.contributor.authorSwierczynska-Krepa, Monika
dc.contributor.authorNiankowska-Mogilnicka, Ewa
dc.contributor.authorHoltappels, Gabriele
dc.contributor.authorDe Ruyck, Natalie
dc.contributor.authorSanak, Marek
dc.contributor.authorDerycke, Lara
dc.contributor.authorVan Crombruggen, Koen
dc.contributor.authorBachert, Claus
dc.contributor.authorPerez-Novo, Claudina Angela
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2018-07-26T17:30:31Z
dc.date.available2018-07-26T17:30:31Z
dc.date.issued2016
dc.description.abstractBackground: Systemic reactions are related to the pathogenesis of Aspirin Exacerbated Respiratory Disease (AERD). With this work we wanted to study the changes in the systemic levels of inflammatory mediators in both baseline and after oral aspirin challenge in patients with and without AERD. Methods: Patients with nasal polyposis and asthma with AERD (n = 20) and without (n = 18) were orally challenged with aspirin in a single-blind placebo controlled study. Serum samples and urine were collected before and 6 h after placebo and aspirin oral challenges. Serum levels of inflammatory mediators were assayed by using the Luminex technology and ELISA. The concentrations of 9-alpha, 11-beta prostaglandin F-2, and leukotriene E-4 (uLTE(4)) were measured in urine samples by ELISA. The expression of T-cell surface markers was analyzed in peripheral blood mononuclear cells isolated before and after the challenges. Results: AERD patients showed significantly higher baseline levels of s-IL-5R-alpha, uLTE(4) and percentage of CD4(+)CD25(+)CD127(pos) and CD4(+)CD45RA(-)CD45RO(+) but decreased levels of TGF-beta(1) and number of CD4(+)CD25(+)CD127(neg) cells. Aspirin challenge induced the release of uLTE4, IL-6 and increased the number of CD4(+)CD45RA(-)CD45RO(+) memory T-cells only in AERD patients but failed to reduce the levels of sCD40L as observed in non-AERD subjects. Further, IL-8 and sIL-5R-alpha levels directly correlated with the PD(20)ASA and the effects of aspirin on IL-6 and number of memory T-cells was more pronounced in subjects showing more strong reaction (bronchial and nasal). Conclusions: AERD patients have a differential baseline inflammatory pattern that supports the role inflammation as underlying mechanism of the disease. Systemic response to oral aspirin challenge was related to an increase in serum IL-6 and the number of circulating memory T-cells in AERD patients. (C) 2015 Elsevier Ltd. All rights reserved.en
dc.description.affiliationHoltappels, Gabriele
dc.description.affiliationDe Ruyck, Natalie
dc.description.affiliationDerycke, Lara
dc.description.affiliationVan Crombruggen, Koen
dc.description.affiliationBachert, Claus
dc.description.affiliationPerez-Novo, Claudina A.] Ghent Univ Hosp, Dept Otorhinolaryngol, Upper Airways Res Lab, Ghent, Belgium
dc.description.affiliation[Pezato, Rogerio] Univ Fed Sao Paulo, Dept Otorhinolaryngol Head & Neck Surg, Sao Paulo, Brazil
dc.description.affiliation[Swierczynska-Krepa, Monika] Medex, Out Patient Allergy Clin, Bielsko Biala, Poland
dc.description.affiliation[Niankowska-Mogilnicka, Ewa
dc.description.affiliationSanak, Marek] Jagiellonian Univ, Sch Med, Dept Med, Krakow, Poland
dc.description.affiliationUnifespDept. of Otorhinolaryngology, Head and Neck Surgery, Federal University of São Paulo, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFlemish Research Board (FWO) [FWO08-PDO-117]
dc.description.sponsorshipFlemish Scientific Research Board (FWO) [A12/5-HB-KH3, 1841713N, G039412N]
dc.description.sponsorshipInteruniversity Attraction Poles (IAP) Project [P7/30]
dc.description.sponsorshipJagiellonian University [K/ZDS/000362]
dc.description.sponsorshipIDThis work was supported by a grant to Dr. Claudina Perez-Novo from the Flemish Research Board (FWO Post-doctoral mandate, Nr. FWO08-PDO-117), grants to Prof. Claus Bachert from the Flemish Scientific Research Board (FWO Nr. A12/5-HB-KH3, FWO mandate: 1841713N and FWO research project: G039412N), the Interuniversity Attraction Poles (IAP) Project P7/30 and the statutory grant from the Jagiellonian University (K/ZDS/000362) awarded to Dr. Monika Swierczynska-Krepa.
dc.format.extent157-167
dc.identifierhttps://dx.doi.org/10.1016/j.cyto.2015.10.011
dc.identifier.citationCytokine. London, v. 77, p. 157-167, 2016.
dc.identifier.doi10.1016/j.cyto.2015.10.011
dc.identifier.issn1043-4666
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/46094
dc.identifier.wosWOS:000366540500020
dc.language.isoeng
dc.publisherAcademic Press Ltd- Elsevier Science Ltd
dc.relation.ispartofCytokine
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectAspirin Exacerbated Respiratory Diseaseen
dc.subjectInterleukin 6en
dc.subjectMemory T-cellsen
dc.subjectOral aspirin challengeen
dc.subjectSystemic reaction after aspirin challengeen
dc.subjectInterleukin 8en
dc.subjectIL-5 receptor alphaen
dc.subjectTGF-beta(1)Sensitive Patientsen
dc.subjectBronchial-Asthmaen
dc.subjectT-Cellsen
dc.subjectModerate Asthmaen
dc.subjectHypersensitivityen
dc.subjectChallengeen
dc.subjectActivationen
dc.subjectTgf-Beta-1en
dc.subjectAssociationen
dc.subjectLeukocytesen
dc.titleSystemic expression of inflammatory mediators in patients with chronic rhinosinusitis and nasal polyps with and without Aspirin Exacerbated Respiratory Diseaseen
dc.typeinfo:eu-repo/semantics/article
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