Effects of Lidocaine, Bupivacaine, and Ropivacaine on Calcitonin Gene-Related Peptide and Substance P Levels in the Incised Rat Skin

Página do item simplificado
dc.citation.issue4
dc.citation.volume29
dc.contributor.authorLapin, Guilherme Abbud Franco [UNIFESP]
dc.contributor.authorHochman, Bernardo [UNIFESP]
dc.contributor.authorMaximino, Jessica Ruivo
dc.contributor.authorChadi, Gerson
dc.contributor.authorFerreira, Lydia Masako [UNIFESP]
dc.coveragePhiladelphia
dc.date.accessioned2020-07-22T13:23:18Z
dc.date.available2020-07-22T13:23:18Z
dc.date.issued2016
dc.description.abstractOBJECTIVE: To evaluate the effect of 2% lidocaine, 0.5% bupivacaine, and 0.75% ropivacaine on the release of substance P (SP) and calcitonin gene-related peptide (CGRP) in skin wounds. DESIGN: A primary, experimental, analytical, prospective, self-controlled, blinded study. SETTING: The study is set in a university research center. INTERVENTIONS: Twenty-eight Wistar rats were randomly divided into 4 groups: lidocaine, bupivacaine, ropivacaine, and the control. After general anesthesia, a local anesthetic or 0.9% saline (control) was injected subdermally along a 2-cm line on the dorsal midline of each rat; 30 minutes later, an incision (nociceptive stimulus) was made along this line. The animals were euthanized, and skin samples were collected from the center of the incision line and sent for CGRP and SP quantification. MAIN OUTCOME MEASURES: Quantification of CGRP and SP by Western blotting. RESULTS: Substance P levels were similar in the lidocaine and ropivacaine groups but were significantly lower than those of the control group (P = .002); no significant difference in SP levels was found between the bupivacaine and control groups. Procalcitonin gene-related peptide levels were significantly lower in the experimental groups than those in control subjects (P = .009), with no significant differences among the experimental groups. No significant differences in CGRP levels were found among all groups. Lidocaine and ropivacaine inhibited SP release. All 3 local anesthetics inhibited the release of procalcitonin gene-related peptide, but not the release of CGRP in rat skin. CONCLUSIONS: Lidocaine and ropivacaine may inhibit neurogenic inflammation by biochemical pathways activated by SP, whereas bupivacaine seems to have no influence on this process.en
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, Div Plast Surg, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Sect Pathol Scars Same Div, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Sch Med, Translat Neurol Unit, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Dept Expt Neurol, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Pathophysiol LIM45, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Study Grp Neuroregenerat, Sao Paulo, Brazil
dc.description.affiliationBrazilian Natl Council Sci & Technol Dev, IB CNPq, Brasilia, DF, Brazil
dc.description.affiliationMED III CAPES Coordinat Improvement Higher Educ P, Brasilia, DF, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, Div Plast Surg, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Sect Pathol Scars Same Div, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt
dc.description.sponsorshipIDFAPESP: 2012/19163-4
dc.format.extent169-177
dc.identifierhttps://dx.doi.org/10.1097/01.ASW.0000480096.01724.91
dc.identifier.citationAdvances In Skin & Wound Care. Philadelphia, v. 29, n. 4, p. 169-177, 2016.
dc.identifier.doi10.1097/01.ASW.0000480096.01724.91
dc.identifier.issn1527-7941
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56159
dc.identifier.wosWOS:000372849000002
dc.language.isoeng
dc.publisherLippincott Williams & Wilkins
dc.relation.ispartofAdvances In Skin & Wound Care
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectNeuropeptidesen
dc.subjectCalcitonin gene-related peptideen
dc.subjectNeurogenic inflammationen
dc.subjectLocal anestheticsen
dc.subjectSubstance Pen
dc.titleEffects of Lidocaine, Bupivacaine, and Ropivacaine on Calcitonin Gene-Related Peptide and Substance P Levels in the Incised Rat Skinen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
EPM - Artigos