Involvement of STAT1 in proliferation and invasiveness of trophoblastic cells

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dc.citation.issue3
dc.citation.volume17
dc.contributor.authorSousa, Francisco Lázaro Pereira de [UNIFESP]
dc.contributor.authorChaiwangyen, Wittaya
dc.contributor.authorMorales-Prieto, Diana M.
dc.contributor.authorOspina-Prieto, Stephanie
dc.contributor.authorWeber, Maja
dc.contributor.authorPhotini, Stella M.
dc.contributor.authorSass, Nelson [UNIFESP]
dc.contributor.authorDaher, Silvia [UNIFESP]
dc.contributor.authorSchleussner, Ekkehard
dc.contributor.authorMarkert, Udo R.
dc.coverageOlsztyn
dc.date.accessioned2020-08-04T13:40:13Z
dc.date.available2020-08-04T13:40:13Z
dc.date.issued2017
dc.description.abstractTrophoblast proliferation and invasion are controlled by cytokines and growth factors present at the implantation site. Members of the Interleukin-6 (IL-6) family of cytokines trigger their effects through activation of intracellular cascades including the Janus Kinase/Signal Transducer and Activator of Transcription (JAK-STAT) pathway. Functions of several STAT molecules in trophoblast cells have been described, but the role of STAT1 remained unclear. Here, potential functions of STAT1 and its activation by. Oncostatin M (OSM) have been investigated in an in vitro model. STAT1 expression and phosphorylation were analyzed in human term placenta tissue by immunohistochemistry. HTR-8/SVneo cells (immortalized human extravillous trophoblast cells) were stimulated with OSM, IL-6, IL-11, Leukemia Inhibitory Factor (LIF) and Granulocyte Macrophage Colony Stimulating Factor. Expression and phosphorylation of STAT1 were analyzed by Western blotting and immunocytochemistry. Fludarabine and STAT1 siRNA were employed for STAT1 depletion. STAT1 transcriptional activity was evaluated by DNA-binding capacity assay. Cell viability and invasion were assessed by MTS and Matrigel assays, respectively. STAT1 was expressed in villous and extravillous trophoblast cells. Low phosphorylation was detectable exclusively in extravillous trophoblast cells. Only OSM and LIF induced phosphorylation of STAT1 in the in vitro model. Challenge with OSM increased cell invasion but not proliferation. Inhibition of STAT1 by fludarabine treatment or STAT1 siRNA transfection reduced cell viability and invasiveness in presence and absence of OSM. These results indicate the potential involvement of STAT1 in the regulation of trophoblast behavior. Furthermore, STAT 1 functions are more efficiently inhibited by blocking its expression than its phosphorylation. (C) 2017 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier Sp. z o.o. All rights reserved.en
dc.description.affiliationJena Univ Hosp, Dept Obstet, Placenta Lab, Klinikum 1, D-07747 Jena, Germany
dc.description.affiliationUniv Fed Sao Paulo, Dept Obstet, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Phayao, Sch Med Sci, Dept Biochem, Phayao 56000, Thailand
dc.description.affiliationHemoctr Unicamp, Rua Carlos Chagas 480,Cidade Univ Zeferino Vaz, Campinas, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Obstet, Sao Paulo, SP, Brazil
dc.description.provenanceMade available in DSpace on 2020-08-04T13:40:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2017en
dc.description.sourceWeb of Science
dc.description.sponsorshipDAAD (German Academic Exchange Service)
dc.description.sponsorshipCAPES (Coordination for the Improvement of Higher Education Personnel) of Brazil
dc.description.sponsorshipGerman Academic Exchange Service (DAAD)
dc.description.sponsorshipIZKF (Interdisciplinary Centre for Clinical Research) at the Jena University Hospital
dc.description.sponsorshipRepublic of Yemen
dc.description.sponsorshipGerman Research Society (DFG)
dc.format.extent218-224
dc.identifierhttps://dx.doi.org/10.1016/j.repbio.2017.05.005
dc.identifier.citationReproductive Biology. Olsztyn, v. 17, n. 3, p. 218-224, 2017.
dc.identifier.doi10.1016/j.repbio.2017.05.005
dc.identifier.issn1642-431X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57379
dc.identifier.wosWOS:000429509900005
dc.language.isoeng
dc.publisherInst Animal Reproduction Food Research
dc.relation.ispartofReproductive Biology
dc.rightsAcesso restrito
dc.subjectTrophoblast cellsen
dc.subjectSTAT1en
dc.subjectSignal transductionen
dc.subjectPregnancyen
dc.subjectCytokinesen
dc.titleInvolvement of STAT1 in proliferation and invasiveness of trophoblastic cellsen
dc.typeArtigo
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