Mitochondrial involvement in carbachol-induced intracellular Ca2+ mobilization and contraction in rat gastric smooth muscle

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dc.contributor.authorCorrêa, Roberta Moreira [UNIFESP]
dc.contributor.authorLafayette, Simone Sette Lopes
dc.contributor.authorPereira, Gustavo José da Silva [UNIFESP]
dc.contributor.authorHirata, Hanako [UNIFESP]
dc.contributor.authorGarcez-do-Carmo, Lúcia [UNIFESP]
dc.contributor.authorSmaili, Soraya Soubhi [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.date.accessioned2016-01-24T14:17:27Z
dc.date.available2016-01-24T14:17:27Z
dc.date.issued2011-11-21
dc.description.abstractAims: Mitochondria are important modulators of Ca2+ homeostasis. However, it is not clear if they modulate and participate in smooth muscle signaling and contraction. the aim of the present work was to investigate the role of mitochondria in Ca2+ transients and contraction induced by metabotropic muscarinic receptor activation in rat gastric smooth muscle.Main methods: Carbachol (CC11)-induced contraction was investigated in the absence or presence of increasing concentration of mitochondrial protonophore, carbonyl cyanide p-(trifluoro-methoxy)phenyl-hydrazone (FCCP), in gastric fundus strips. Ca2+ and mitochondrial membrane potential (Delta Psi m) measurements were performed in primarily cultured gastric smooth muscle cells loaded with FURA-2 or TMRE dyes.Key findings: Results show that CCh (1 mu M)-induced contraction was inhibited by FCCP in a concentration-dependent manner. in cultured smooth muscle cells CCh (1 mu M) caused a cytosolic Ca2+ rise. Preincubation with FCCP strongly inhibited CCh-evoked Ca2+ transients indicating that mitochondria shape intracellular Ca2+ Signals. CCh induced elevations of Delta Psi m in 60% of the individual mitochondrion analyzed.Significance: Taken together our results indicate that CCh induces release of Ca2+ from intracellular stores, which may be modulated by mitochondria. Thus, mitochondria participate of the intracellular Ca2+ homeostasis in muscarinic contraction in gastric fundus smooth muscle. (C) 2011 Elsevier Inc. All rights reserved.en
dc.description.affiliationFed Univ São Paulo UNIFESP EPM, Dept Pharmacol, Paulista Sch Med, São Paulo, Brazil
dc.description.affiliationFed Univ Pernambuco UFPE, Dept Pharmaceut Sci, Recife, PE, Brazil
dc.description.affiliationUnifespFed Univ São Paulo UNIFESP EPM, Dept Pharmacol, Paulista Sch Med, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent757-764
dc.identifierhttp://dx.doi.org/10.1016/j.lfs.2011.08.003
dc.identifier.citationLife Sciences. Oxford: Pergamon-Elsevier B.V., v. 89, n. 21-22, p. 757-764, 2011.
dc.identifier.doi10.1016/j.lfs.2011.08.003
dc.identifier.fileWOS000296932100002.pdf
dc.identifier.issn0024-3205
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/34231
dc.identifier.wosWOS:000296932100002
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofLife Sciences
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectCalciumen
dc.subjectMitochondriaen
dc.subjectSmooth muscleen
dc.subjectCarbacholen
dc.subjectContractionen
dc.titleMitochondrial involvement in carbachol-induced intracellular Ca2+ mobilization and contraction in rat gastric smooth muscleen
dc.typeinfo:eu-repo/semantics/article
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