Chondroitin Sulfate Impairs Neural Stem Cell Migration Through ROCK Activation

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dc.citation.issue4
dc.citation.volumev. 55
dc.contributor.authorGalindo, Layla Testa [UNIFESP]
dc.contributor.authorMundim, Mayara T. V. V. [UNIFESP]
dc.contributor.authorPinto, Agnes S. [UNIFESP]
dc.contributor.authorChiarantin, Gabrielly Maria Denadai [UNIFESP]
dc.contributor.authorAlmeida, Maira E. S.
dc.contributor.authorLamers, Marcelo L.
dc.contributor.authorHorwitz, Alan R.
dc.contributor.authorSantos, Marinilce F.
dc.contributor.authorPorcionatto, Marimélia Aparecida [UNIFESP]
dc.coverageTotowa
dc.date.accessioned2020-07-20T16:31:13Z
dc.date.available2020-07-20T16:31:13Z
dc.date.issued2018
dc.description.abstractBrain injuries such as trauma and stroke lead to glial scar formation by reactive astrocytes which produce and secret axonal outgrowth inhibitors. Chondroitin sulfate proteoglycans (CSPG) constitute a well-known class of extracellular matrix molecules produced at the glial scar and cause growth cone collapse. The CSPG glycosaminoglycan side chains composed of chondroitin sulfate (CS) are responsible for its inhibitory activity on neurite outgrowth and are dependent on RhoA activation. Here, we hypothesize that CSPG also impairs neural stem cell migration inhibiting their penetration into an injury site. We show that DCX+ neuroblasts do not penetrate a CSPG-rich injured area probably due to Nogo receptor activation and RhoA/ROCK signaling pathway as we demonstrate in vitro with neural stem cells cultured as neurospheres and pull-down for RhoA. Furthermore, CS-impaired cell migration in vitro induced the formation of large mature adhesions and altered cell protrusion dynamics. ROCK inhibition restored migration in vitro as well as decreased adhesion size.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Biochem, Neurobiol Lab, Rua Pedro de Toledo 669,3 Andar, BR-04039032 Sao Paulo, SP, Brazil
dc.description.affiliationButantan Inst, Physiopathol Lab, BR-05503900 Sao Paulo, Brazil
dc.description.affiliationUniv Fed Rio Grande do Sul, Dept Morphol Sci, BR-90050170 Porto Alegre, RS, Brazil
dc.description.affiliationUniv Virginia, Dept Cell Biol, Sch Med, Charlottesville, VA 22903 USA
dc.description.affiliationUniv Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, BR-05508000 Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biochem, Neurobiol Lab, Rua Pedro de Toledo 669,3 Andar, BR-04039032 Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa de Sao Paulo - FAPESP [2011/00526-7, 2012/00652]
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico - CNPq [404,646/2012-3]
dc.description.sponsorshipNational Institute of General Medical Sciences [GM23244]
dc.format.extent3185-3195
dc.identifierhttp://dx.doi.org/10.1007/s12035-017-0565-8
dc.identifier.citationMolecular Neurobiology. Totowa, v. 55, n. 4, p. 3185-3195, 2018.
dc.identifier.doi10.1007/s12035-017-0565-8
dc.identifier.fileWOS000427097500037.pdf
dc.identifier.issn0893-7648
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55794
dc.identifier.wosWOS:000427097500037
dc.language.isoeng
dc.publisherHumana Press Inc
dc.relation.ispartofMolecular Neurobiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectNeural stem cellen
dc.subjectCell migrationen
dc.subjectChondroitin sulfateen
dc.subjectTraumatic brain injuryen
dc.subjectRhoAen
dc.subjectRocken
dc.titleChondroitin Sulfate Impairs Neural Stem Cell Migration Through ROCK Activationen
dc.typeinfo:eu-repo/semantics/article
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